15 Hot Plasmids from 2017
Type
Blog Post
Published
Jan. 9, 2018, 3:02 p.m.
... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene.
Koo...fusion protein retained the PAM properties of the individual Cas9. Kim et al. also mutagenized the cytidine...proposed to be essential in B. subtilis 168. Each individual strain includes a xylose-inducible dCas9 paired...