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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 58 results for: gag pol

Showing: 41 - 58 of 58 results

  1. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  2. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...relevant parties, and the company at large, by polling and informal communications we made a decision ...scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (multiple choice/binary...scientists of all types to stay up to date and engaged with new advances in published science. They also...

  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...to solve a problem. But how do you intentionally engage in a creative problem-solving process?  You may...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility... posts on the defunct genome editing technology NgAgo). In addition, there are organizations like ReFigure.... Down the line we may see further adoption of policies that promote reproducible publishing practices...

  5. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ... your experiment is definitely not a fluke.” 3. Engage in various projects and help colleagues with their...expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD ...

  6. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ... both knowledgeable in their field and eager to engage with the work of others. Having an environment ... with my teammates acted as my safety net and trampoline all at once. I was able to do something that ...

  7. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...be other scientists, or science journalists, or policy makers. The term editor covers a wide variety of...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ...

  8. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...initially limit the technology to research centers engaged in long-term, mandatory follow-ups and observational...recognize so-called bad actors among them and “self-police” or whether the media and academic conference response...

  9. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...or contract, you should be starting to send out polite emails with your CV to people you’d like to work...scientists and technical people) is linked to engagement and access to growth opportunities, not salary...

  10. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ... from audiences as they do science outreach and engage with the public, and learn from the process of ...broader impacts for their work in education and policy and to draw attention and support to work in their...

  11. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...Editing the PAM prevents the prime editor from re-engaging with DNA it has already edited. Created with BioRender.com... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

  12. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...engineered SpCas9 variants with alternative PAMs like NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

  15. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...sharing cells: the black market of cell biology. Engaging in this practice would seem to save time and money...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

  16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...When these molecules, called Bispecific T-cell engagers (BiTE®s), bind CD3 on T cells and a tumor-specific...

  17. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...lab Writes a lot of grants Varying degrees of engagement with what happens in the lab Official title is...dedicated to different career options from science policy to science communication and much more. I hope ...

  18. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... site and contains the desired edit (or edits) engages the prime editor protein. The prime editor consists...transcriptase domain copies the desired edit, directly polymerizing new DNA onto the nicked target strand. The initial...
Showing: 41 - 58 of 58 results