...Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide Protocols...Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your gRNA for CRISPR... that made it easy to share plasmids, both for people depositing plasmids and for people requesting them... Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression... Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid? How to Design...video, however, is “What is a Plasmid?”, An animated explainer of a plasmid and its parts that helps the... was only after creating a plasmid repository and shipping internationally, after all, that we could identify...

...construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are ...index.html Design crRNA/ssODN: Using Ensembl Transcript ID(s) as an input query, pick crRNA (guide RNA sequence...two-part cr:tracrRNA system is much more potent than the in vitro-transcribed single guide RNA (IVT sgRNA...there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure 2: Cas9... developed a “TAG-IN” bioinformatics tool for guide RNA and donor DNA design, and implemented our optimised...successful delivery of three reagents to cells: a guide RNA, Cas9 protein, and a donor DNA that contains the...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did...with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating Cas9, fused... al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al. (2023). Efficient engineering...inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C., Goeden...