... Rinehart lab synthesized the activated form of human mitogen-activated ERK activating kinase 1 (MEK1)...the mitochondrial targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported...pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP...N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and plant resistance... The strategy relies on several observations: Rapamycin is a membrane-permeable pharmacological agent ...induction heterodimerization of proteins that contain rapamycin-binding domains, such as FKBP and FRB. The cell...membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) and ...

...ti2learning.org. References 1. Dieterich, Daniela C., et al. "In situ visualization and dynamics of newly...Nathans, Daniel. "Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains...attended a very interesting talk by Prof. Erin M Schuman, a former Caltech researcher, now Director at the...newly synthesized proteins with FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical...the turnover of one specific protein. Professor Schuman’s lab showed that FUNCAT’s usefulness can be extended...interaction. To study protein levels in situ, the Schumann lab used a modified version of PLA where one antibody...Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings...