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We narrowed to 62 results for: lentiviral construct

Showing: 41 - 60 of 62 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...generating a series of single- and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf... expression and labeling approach using these constructs in HEK 293 cells (Smith et al., 2024). They then... license.  The team deposited an array of constructs for tagging the kinesins KIF5A and KIF5B as well...Kv2.1 targeting motif as well as a pan-membrane construct without the Kv motif. Altogether, if you want ...proportions from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). ...

  2. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...One good system to balance these needs is the lentiviral-based expression of floxed dCas9 so that it may... for cells stably and highly expressing these constructs can be achieved by fluorescence-activated cell... stable gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin...

  4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...from three to two. Lentiviral delivery of BioTAP-tagged dCas9 and gRNAs. Using lentiviral delivery of the...  A second application for CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction... BirA, and 3) one or more gRNAs Two separate lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...microtubule severing. A) Schematic of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and ...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line...is optogenetically recruited by different CIB constructs to the plasma membrane or lysosomes (the earlier...earlier article also provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4)...100x variants. But those super-high efficiency constructs are great for in vitro biocatalytic applications...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo tools can...gene-of-interest, all the way to the complex multi-TU constructs that compose genetic circuits and metabolic pathways...MoClo is commonly used to design TUs and multi-TU constructs for synthetic biology applications in bacteria...individual TUs or very large (up to 20!) multi-TU constructs (Moore et al., 2016). The variety of constitutive...multiple unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for...is geared towards cloning multi-TU expression constructs (Martella et al., 2017). If you can dream up ...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...

  7. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed Addgene...

  8. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable...efficiency was not affected by CellTagging. By constructing lineage trees and comparing them with the gene...reprogramming and differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101...

  10. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well... with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy...PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral...

  11. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...was rapidly able to support sharing of CRISPR constructs and information across the globe.  “Read the ...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing...

  12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...in women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these...on building upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert! ...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...cell types, while shRNA and siRNA are synthetic constructs introduced through external methods. shRNA processing...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...C. (2006). Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnology,...

  15. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...selectable marker. This vector can express your construct in target cells transiently or constitutively ...Some customization is required, but many viral constructs have already been built and are available on ... embryonic stem cells. Viral integration of a construct is also reliably higher than traditional transfection...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

  16. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...the design and construction steps are complete, it’s time to make virus! The constructed viral vector containing...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

  17. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

  18. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...integrated by the virus. If an oncogene overexpression construct is being delivered, that is a higher risk cargo...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...you have to work with BSL-2 materials, such as lentivirus. Most of this work will need to be done in a ...that particles are not sprayed into the air. For lentivirus and other BSL-2 materials, you’ll need to use...for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge bin covers, which...

  19. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...justifying each single element used to build the construct and explain why they selected a certain target...has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...

  20. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...sequences can be used together in DAP array. To construct DAP array for multiplex gene editing applications...deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes ...
Showing: 41 - 60 of 62 results