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We narrowed to 68 results for: lentiviral production

Showing: 41 - 60 of 68 results

  1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...via a lentiviral vector to cell lines that either stably expressed Cas9 or that had been lentiviral infected...regulation, including almost all genes involved in the production of a cell surface glycan that’s required for ...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

  2. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...company.  Ultragenyx’s goal is to develop novel products for the treatment of rare and ultra-rare diseases...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...

  3. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's 10 year anniversary...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing... recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill a community...

  4. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...genus capable of producing a variety of natural products. Recently, this group optimized their initial ... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

  6. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...-studied lentivirus is HIV, which many lentiviral tools have been derived from. Lentiviruses have slightly...adenovirus has the highest maximum titer, a plus for production and infection. This virus can infect dividing... with immortalized cell line cultures. Lentivirus Lentivirus is a type of retrovirus and shares many ... commonly used lab viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus...gamma-retrovirus, coming in around 9 kb. Unlike gamma, lentivirus can infect both dividing and non-dividing cells...nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related, ...specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering...

  7. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...precursors, biofuels, and natural products without the undesirable side products generated during chemical synthesis...these molecules. To make large amounts of pure product in an affordable way, researchers test many different... resistance through gene stacking, increasing production of a protein of interest, and improving the nutrient...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors.” ACS Synthetic...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Saccharomyces cerevisiae, as a key host for the production of their products, including fermented beer and wine, ...the authors packaged it into lentivirus and used the resulting lentiviral library to generate a population...Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable...Library. This complex second-generation CRISPR lentiviral library targets nearly all human protein-coding...ingredients, and recombinant proteins. In order to keep production yields high and costs low, and to deal with the...from the CDC and cloned into a third generation lentiviral transfer vector. Each gene has been FLAG-tagged...applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...., BMC Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems...Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants...purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from ...direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck Institute of Biochemistry...upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...Quality control for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007...2016). Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular...

  11. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...mature siRNA duplex. Fun fact! Endogenous miRNA production starts with transcription of the miRNA to create...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...

  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...risk is high. You are generating a gene therapy product with CRISPR to (hopefully) be used in human clinical...been used - GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the...

  14. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...sites including both the edit rate and the repair product sequence. Targeted amplicon sequencing doesn’t ...CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

  15. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... with these tools, I found myself writing AAV production tricks into the margins of my protocol. While...life easier!  In this post, I’ll share some AAV production, purification, and titration tips, while also... and analyses needed for packaging AAV. AAV production Overview: The first step in packaging AAV is ...expand the HEK293 cells. Check out the Addgene AAV Production protocol for more details. Pro tips I’m not ... have enough AAV. Compared to the Addgene AAV Production protocol, I take a lazier approach for harvesting...then the viral suspension generated during AAV production is overlaid. Following ultracentrifugation, the...AAV plasmid that was carried over from the AAV production process. Either SYBR green technology or a TaqMan...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...diligently throughout the production process.  Helper-free production Unlike lentivirus, AAV does not possess...For more details on the production process, check out the Addgene AAV Production protocol. Titering Once...experience. Here, we’ll go over the basics of rAAV production.  Of course, you will want to make sure that...facilitate efficient replication. The most common production system, however, is a “helper-free” system, in...).    Figure 1: Overview of key steps of AAV production, iodixanol gradient purification, and AAV titration...remove residual plasmid DNA carried over from the production process. For more details on the qPCR titering... capsid ELISA: Packaging of genomes can limit production of recombinant AAV-2. Gene Therapy, 6(7), Article...

  17. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...along with viral packaging plasmids into a viral production cell line, usually HEK-293 cells. When all of...these viral plasmids are expressed within the production cell line, viral particles will be produced and...Typically, one day after transfection into the production cell line (depending on the virus), virus can...Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus?...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

  19. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection of all three plasmids... into a producer cell line, such as HEK-293T, production of viral particles, and transduction of a target...centrifuging viruses (a necessary step during production), there is a significant risk for aerosol contamination...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...you have to work with BSL-2 materials, such as lentivirus. Most of this work will need to be done in a ...
Showing: 41 - 60 of 68 results