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Showing: 41 - 49 of 49 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...just one at a time. Many of these libraries are packaged and delivered with viral vectors instead of a ...

  4. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...(A) signals in combination with certain viral packaging systems has been associated with reduced viral... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...other viral vectors such as adenovirus (AdV) and lentivirus (LV) are known to transduce retinal cells, AAV...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes....

  6. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...published a protocol and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited... are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...here). To use the library, the authors packaged it into lentivirus and used the resulting lentiviral library... of dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing...applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed... system has a much larger cargo capacity than lentivirus and is capable of efficiently creating stable...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral...PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. ...

  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...between the transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...
Showing: 41 - 49 of 49 results