We narrowed to 49 results for: lentivirus packaging
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TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...just one at a time. Many of these libraries are packaged and delivered with viral vectors instead of a ...
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Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....delivered into the brain region of interest. AAV or lentivirus? Both AAV and lentiviral vectors are used for...the other hand, lentiviral vectors are superior packaging vectors for larger genes - they can hold up to... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....(A) signals in combination with certain viral packaging systems has been associated with reduced viral... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....other viral vectors such as adenovirus (AdV) and lentivirus (LV) are known to transduce retinal cells, AAV...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes.... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....published a protocol and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited... are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....here). To use the library, the authors packaged it into lentivirus and used the resulting lentiviral library... of dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing...applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed... system has a much larger cargo capacity than lentivirus and is capable of efficiently creating stable... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral...PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. ... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....between the transposon’s LTRs, similar to when packaging viral vectors. Let’s take a closer look at three... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...