We narrowed to 111 results for: max

...growth time, temperature, and shaking speed to maximize cell density and plasmid yield as these factors...need to be grown for 20 hours or more to achieve maximum plasmid yield. The optimal growth time should be...

...dilution series of the standard and the minimum and maximum detection limits of the instrument being used to...specificity, especially as it approaches the minimum and maximum thresholds. If the measurements for the unknown...

...authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this ...plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits ...

...s the maximum length of codons that can be mutated? 88 codons, or 264 basepairs, was the maximum length...

...bloggers Dominik Paquet and Dylan Kwart from Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne...Institute for Stroke and Dementia Research, Ludwig-Maximilians-University Munich. His lab develops iPSC-based...

...s illumination (see Figure 2). H describes the maximum signal strength, while W results from the time ... The resulting histograms is described through maximum current (H), the time the cell needed to pass through...

... guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR efficiency. Easi-CRISPR is compatible... as possible to your desired insertion site to maximize HDR efficiency. In this case, we’re fortunate ...

...acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional allele...Selection of gRNAs for an experiment needs to balance maximizing on-target activity while minimizing off-target...

...population level, but each individual cell only has a maximum of one gene knocked out. In this genetically heterogeneous...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

...UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier name CGBE1, for....2% of studied sequences) (Huang et al., 2024). BE4max, a CBE, generated similar deletions at a lower ...

...partners, and the larger scientific community to maximize the positive impact that science can have on the...

...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

...gossip here – this means encouraging a culture of maximum information flow so everyone knows what is going...

...academia How can we shape our time in academia to maximize productivity and success? We asked leading professors...

...potassium-selective channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm and two-photon excitation ...

...10 gRNAs per gene. The guides are optimized to maximize on-target cleavage and minimize off-target effects...

...available as soon as your paper is published helps maximize the potential impact of your work. Plus, it’s ...

...natural evolution would produce transposons with sub-maximal efficiency, and this barrier could be overcome ...

...intelligent discussion, and/or brainstorming sessions to maximize productivity and direction of the project? What...

...2011 Jun; 9, 467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432...