...nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control...Figure 5: A) Representative confocal images showing GFP expression from ssAAV5s with hIBA1 or hIBA1a promoters...Quantifications showing high microglia specificity of GFP expression for the indicated ssAAV5s. C) Quantifications...

...line, Hep3B. From left to right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay).   ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...(DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses..., Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore...the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its fluorescence properties...

...sensors available that will localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an ...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...

...red light instead of blue to excite a molecule of GFP. Because red light has approximately half the energy...light, two photons of red light are needed to excite GFP compared to only one of blue. Only at the focal point...detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment,...infinitely small point of light – think of a single GFP molecule – will appear as a fuzzy 200-300 nm spot...

...overlap. Figure 4 compares the spectra of enhanced GFP (eGFP) and FITC, the latter being a commonly used fluorophore...to fuse them with a fluorescent reporter, such as GFP or mCherry, through genetic modification. As your... you can also use fluorescent reporters, such as GFP, and fluorophore-conjugated antibodies together in...expression, CD45 is incorporated into the plasma membrane and can be detected through a cell surface label...vector also encodes for eGFP, which serves as a transduction reporter. Note that eGFP is not fused to CD45...-Cy7. Next comes our transduction reporter, eGFP. As eGFP emits fluorescence by itself and does not need...cytometer which channel to use to detect eGFP. As you saw in Figure 4, eGFP strongly overlaps with FITC, hence...

...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,... (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA...

...in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...

...containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA...liposome) or electrical (electric current forming membrane pores) transfection. These techniques are still...viral titre to the number of cells transduced by a GFP encoded virus, and the reverse transcriptase activity...

...derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable...

...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...the background. Dyes that can pass through cell membranes can prove useful for live-cell imaging. Experimental...receptors. In Biochimica et Biophysica Acta - Biomembranes (Vol. 1838, Issue 1 PARTA, pp. 15–33). https...

...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...workshops. Let’s use TIM-1 (Single pass type-1 membrane protein), which has been designated CD365 in the...