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We narrowed to 98 results for: mouse c rel

Showing: 41 - 60 of 98 results

  1. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable to other...labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling of the...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal ...Feng Q, Zhou Y, Zeng S, Ren M, Jiang S, Ni H, Zhou C, Gong H, Luo M (2018) Cell-type-specific and projection-specific...these labor-intensive and invasive techniques to reliably label any post-mitotic cells in Drosophila. SPARC... be labeled in a given population is directly correlated to the length of the truncated attP site. The...

  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... one gene and activate another. In the field of mouse genetics, FLEx is currently being used to study ...really due to a single gene deleted in your knockout mouse, you can design a FLEx switch conditional knock-...genOway press release for more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling...Cre-mediated recombination at the cellular level in the mouse. 2003 PubMed PMID: 12665802. 2. Branda, CS and Dymecki...

  3. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...anti-mouse CD11b-BV510), which is present on dendritic cells but not T cells, and CD8 (anti-mouse CD8b-PerCP-Cy5.5...experiment. Practical example: lymphocytes in a mouse tumor sample You made it! So much about the theoretical...where I harvested a melanoma tumor from a C57BL/6 mouse and analyzed the tumor-infiltrating lymphocytes ...Using a practical example of lymphocytes from a mouse tumor, you’ve learned how to read an FSC/SSC plot...in fewer cells being considered for the analysis. (C) A more liberal gate allows more cells to be considered...looser gate here. I am not worried about including irrelevant events in my analysis, as these will get excluded...from your single cells, as duplicates can’t be reliably analyzed on their staining signals. For this, ...

  4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...transmission in brain slices from mouse, and showed that it can alter motor responses in C. elegans. Find the split... present, it will repress expression of mKate2 relative to EBFP2. Weiss’s group used these reporters to...chain of BoNT/B into two fragments (BotNT-N and BotNT-C) and joined each fragment with one part of an established... it inactive, thus inhibiting neurotransmitter release. How is this light-mediated? The researchers split...visualizing the location and function of microbes rely heavily on fluorescent protein genes. These optical...

  5. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...ideal for whole animal administration, such as in mouse experiments. AAV also has multiple serotypes (12...induce a lower inflammatory response within hosts. Relative to the other viruses discussed in this article...10.1016/j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...2011 Jun;3(6):677-713. DOI: 10.3390/v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of...lentivirus and gamma-retrovirus are so closely related, their required components are very similar. While...

  6. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov, R., Fort, A., Gebhard, C., Goldowitz, D., Guhl,...Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998). Potent and specific genetic interference...raw %}{"no_follow":false,"open_in_new_tab":true,"rel":"noopener","sponsored":false,"url":{"content_id"...atlas of miRNAs and their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878. https://doi.org...10.1038/sj.emboj.7600385 McIntyre, G. J., & Fanning, G. C. (2006). Design and cloning strategies for constructing...6750-6-1 O’Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms....1080/15476286.2023.2288741 Taxman, D. J., Moore, C. B., Guthrie, E. H., & Huang, M. T. (2010). Short ...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...another popular model organism. A close relative to the mouse, both are great mammalian models due to .../000504294 Wang, T., Guan, C., Guo, J., Liu, B., Wu, Y., Xie, Z., Zhang, C., & Xing, X.-H. (2018). Pooled...that make them an ideal model organism; they are relatively simple to maintain and breed and they have large...doi.org/10.13918/j.issn.2095-8137.2016.4.237 McCusker, C., & Gardiner, D. M. (2011). The Axolotl Model for ...elife.50651 Vieira, W. A., Wells, K. M., & McCusker, C. D. (2019). Advancements to the Axolotl Model for ...BSL-1 status, E. coli can be used as a model to relate these processes in more pathogenic bacteria (Blount...regeneration.  In addition, axolotls have many age-related phenotypic changes that are similar to those observed...

  8. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...organisms and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations...Importantly, since Protein A has weaker affinity for mouse IgG, it is helpful to incubate with a rabbit secondary...digestion, fragments are released from nuclei by a short incubation at 37 °C. These short DNA fragments...or more transient DNA-protein interactions, the reliance on fixation does increase the chance of false-..., which stands for ‘Cleavage Under Targets and Release Using Nuclease,’ was recently developed by the ...strong detergent to permeabilize cells rather than to rely on nuclei extraction. They report that this change...

  9. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...throughout the brain. Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive....2019.02.037 Kagiampaki, Z., Rohner, V., Kiss, C., Curreli, S., Dieter, A., Wilhelm, M., Harada, M., Duss...References and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H.,...inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors...

  10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...these tools because obviously, unlike the mouse, we can’t rely on genetic engineering to optimize humans...to fuse the activators instead of at the protein’s C terminus. With that new design in place, Konermann...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki...Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann ... not managed to create a system to allow us to reliably activate essentially any gene,” she says. The ...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Park, J. C., Zhao, J., Luebbers, A., Green, E., Philibert, C. E., Zhang, H., Layne, M. D., Wu, J. C., & Garcia-Marcos...AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal antibodies targeting the human genes ...including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf tag; see Figure 2), the authors developed...human stem cell-derived neurons expressing ASAP5-Kv. C) Cultured rat hippocampal neuron expressing pan-membrane...the soma. The initiation site identified in panel (C) is indicated; red line is exponential fit. Adapted...the self-labeling tags SNAPf and CLIPf. These tags rely on synthetic dye molecules, which are much brighter...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...shown to have a half life of 84 hours in primary mouse embryonic fibroblasts. This fusion protein can be... synaptic proteins Fluorescent protein system for C. elegans Fluorescent timers for studying cell cycle...native locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since it requires...can you use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet...together, they fuse and fluoresce. The team engineered C. elegans cell lines to express wrmScarlet1-10 unattached...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...human glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library at ...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    .... Speed: AAV transduction experiments are relatively quick. We have found that expression occurs quickly...at (striatum, hippocampus and cortex) within the mouse brain (4). An example from the cortex is outlined...transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain." PloS one 8.9 (2013): e76310. PubMed PMID...nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be used quickly...PubMed Central PMCID: PMC190186. 9. Grieger, Joshua C., and Richard J. Samulski. "Packaging capacity of ...roles of certain cell types in locomotion. We have relied heavily upon AAV vectors to deliver chemogenetic...AAV selection, there are three important factors related to location. Location (1): What tissue? Location...

  14. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ... use of the LITEs to control gene expression in mouse neurons and in the brains of living mice both quickly...groups making use of the new optogenetic tools in C. elegans and in Plasmodium. For those who do want ...those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A ...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...

  15. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...M, Hu G, Fang F. Fudenine, a C-terminal truncated rat homologue of mouse prominin, is blood glucose-regulated...You know qPCR is most commonly used to measure relative quantification of mRNA, which can be used as a...reported to work well in the Lightcycler while being relatively easy to multiplex (Thellwell, 2000). See Table... Accuracy is not as important as precision for relative quantification. A consistent 5% positive error...Accurate and statistically verified quantification of relative mRNA abundances using SYBR Green I and real-time...150 bp long Annealing temperature should be 60-65°C For qPCR, the important primer variable is the annealing... the primers.  Melting temperature should be ~60°C (between 50 and 65, with no more than 3 degrees between...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...4355596 King, S. R., Duggal, N. K., Ndongmo, C. B., Pacut, C., & Telesnitsky, A. (2008). Pseudodiploid Genome...Pugh, W. E., & Teich, N. (1971). Noninfectious ARK mouse embryo cell lines in which each cell has the capacity...34372494. Prussin, A. J., Garcia, E. B., & Marr, L. C. (2015). Total Virus and Bacteria Concentrations in...Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated into HIV...tools in molecular biology. “Retroviruses” is an umbrella term which encompasses 1.) the gamma-retroviruses...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...The genome of viral tools are often not virology related at all, and you will instead get expression of ...

  17. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ... and achieved therapeutically relevant editing efficiencies in the mouse brain, liver, retina, heart, ... a mutation that causes Niemann-Pick disease type C, directly demonstrating the therapeutic potential ...toxicity in long-term experiments.  Figure 4: The C-terminus of Cre (CreC) and the N-terminus of Cre (...Physiol 2018. PubMed PMID: 30819783. Light up CreLite: a red-light inducible optogenetic tool for precision...lineage tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool...systems (ex: tamoxifen). As the name suggests, CreLite combines the Cre/lox system with optogenetics. ...studies. Eisenhoffer and his colleagues evaluated CreLite for cell tracing using the transgenic zebrafish...

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...fourth-generation base editors, BE4, reduce the undesired C→G or C→A conversions that can happen with BE3s. These...Compared to BE3, BE4 offers a 2.3 fold decrease in C→G and C→A products as well as a 2.3 fold decrease in indel... Hong, M., Zhang, D., Luan, C., Zhang, S., Wu, H., Gao, H., Bai, S., Li, C., Ding, R., Xue, N., Lei, Z... W., Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt...non-homologous end joining (NHEJ), HDR occurs at a relatively low frequency, especially in nondividing cells...particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation cytosine base editors...) and adenine base editors (ABEs). CBEs mediate a C to T change (or a G to A change on the opposite strand...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...Yamada, K., Kuznetsov, I. A., Rand, E. A., Ochs, C. C., Jaber, Z., Gardner, K. H., & Chow, B. Y. (2022...eLife.84364.  Meiring, J. C. M., Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. (2022)....activities like neuronal signaling — think of a mouse with an LED brain implant or a worm wiggling back..., N., Ononiwu, F., Pal, D., Manikas, J., Taveras, C., Hall, N. A., Silva, J. D., Freshour, J., & Hehnly...j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed ...roles played by microtubules, Opto-katanin will surely be useful for a wide variety of experiments. Opto-RhoGEFs...
Showing: 41 - 60 of 98 results