We narrowed to 58 results for: psin

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...they wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can...

2. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator...

3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ... also successfully regulated expression of channelrhodopsin-2, commonly used in optogenetics, opening ...

4. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ... purified via streptavidin pulldown. Following trypsin digest, PPIs are identified via mass spec analysis...

5. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ...a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now available as AAV5. Find the AAV...

6. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...

7. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

8. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...

9. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...

10. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ... place the needle in the correct vein without collapsing it. Retro-orbital injections are also thought...

11. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...they determined that an A122D mutation in the rhodopsin domain conferred several new improvements. The...

12. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ... M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range...

13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...blue-light controlled optogenetics, such as channelrhodopsins and tools based on light-oxygen-voltage (LOV...

14. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...

15. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the...

16. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Selective promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or...

17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...short expression times, where as others, such as hSynapsin, are relatively weak and may require longer waiting...

18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...uses genetically encoded tools, such as microbial opsins, to control cellular activities using light. In...