We narrowed to 76 results for: quo;
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TypeBlog PostPublishedJune 1, 2017, 2:30 p.m.... et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a reference protein is available...
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The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....or build on work done previously, borrow small aliquots of restriction enzymes for one-off projects, or... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....photostable protein for live cell imaging would be mTurquoise2, based on the data acquired at low excitation... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m.... and catalog numbers. As we are happy to share aliquots of dTAG-13 in the meantime, please address your... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....related to the structure of the biomedical workforce. Quotes taken directly from participants (summarized in... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just part of... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....Barrera LA, Born DA, Edwards A, Gehrke JM, Lee S-J, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....delivery may also be achieved via injection into the liquor of the cerebral ventricles (ICV, intracerebroventricular... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....freeze-thaw cycles and should be stored in single-use aliquots. Never use a polybrene solution that has been ... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....long term use, you may want to consider using an aliquot that has been subjected to a freeze/thaw cycle ... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....plasmid diversity, so Addgene strives to distribute aliquots of the original library whenever possible. In ... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....starting off with a new cell line, grow the cells, aliquot them into a number of vials, freeze them, and store... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....Fluorescent Protein (GFP), a protein from the jellyfish Aequorea Victoria. Since that discovery, the family of ... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m.... from where you prepare your sample dilutions. Aliquot all of your reagents into single-use tubes and ... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing buffer, polymerase...