We narrowed to 78 results for: sas;
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TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....Types of ELISA There are four different types of ELISAs, which differ in the way the antigen is detected...available for your antigen as the other types of ELISAs require two different antibodies. As mentioned ...
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Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....bypassed by leaving out Cas3 — a tactic some CRISPR transposase systems utilize with type I-F Cascades. ...Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines, it is better to first engineer...the mARG cassettes 2:1 (A:N-V) with PiggyBac transposase and outgrow the cells for a several generations... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022), that overcomes... optogenetic tool for localized, microtubule disassembly. Current Biology, 32(21), 4660-4674.e6. https... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....in the experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked...in a series of samples, such as Western blots or ELISAs, or for other experiments to study protein-protein... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m..... Type I systems are also employed as CRISPR transposases, by leaving out Cas3. Type I is further subdivided...paradoxically called V-K, is a CRISPR-associated transposase (CAST) that can insert large fragments of DNA... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry... -
CRISPR Guide Update
TypeBlog PostPublishedOct. 10, 2024, 1:15 p.m....CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m....Cytology group! @EikeMahlandt @AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog PostPublishedAug. 17, 2021, 1:15 p.m.... We have GIFs for every occasion. Disaster in the lab you haven’t quite gotten over? We have... -
2018 #DeckTheLab Contest Winners
TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk @ShebaAJ pic.twitter.com/X6QYXteRLT... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C but preferentially... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog PostPublishedMarch 7, 2019, 2:39 p.m.... which we now realize is a major environmental disaster. We should continue to examine our behavior and... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....don’t generally affect antibody applications like ELISAs or Western blots, they are key in helping antibodies... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....advantage but error-prone repair of the gene would be disastrous (i.e. if breaking the gene is worse than leaving... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses can accommodate... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting. The five isotypes of antibodies... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m.... Lastly, once a mutant is identified, the PB transposase can easily and seamlessly remove the integrated... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....densely populated places like the University of Kansas and the University of Louisville in Kentucky, the... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m....stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity to light, and better...