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...’t give up! Rather than plotting the entire data set, plot only the linear portion. This allows you to...standard curve. Take the following precautions to set yourself up for success!  Study the instruments ...see for the unknowns in your experiment. Carefully set up a standard curve dilution series that takes into...curve and a positive control in every run.  When setting up your dilution series, use consistent dilution...

...vectors perform in various experimental settings. It is a curated set of technical reports on AAV vectors ...our target neurons (Figure 1). However, this one set of experiments didn’t provide any information on ...10.1089/hgtb.2015.040 Initiative, F. B. (2019). Setting your data free. Nature, 5–7. O’Connor, D. M., Lutomski...tested four serotypes containing an expression cassette with an ubiquitous promoter to express GFP in ...

...relationship is extremely important – without a good set up it could be very difficult for you to become proficient...lot to contribute to the relationship dynamic and set yourself up for success! Getting the most out of ... would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...Remember, your bench mentor’s training time is time set aside just for you – so do your best to show up as...

...our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins, and...existing backbones, we're excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones...stay tuned for mammalian expression and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral...for Protein Innovation (IPI) recently released a set of highly-specific recombinant antibodies against...

... management involves a different skill set: mentoring, setting strategic goals, and fostering team success...Miscommunication often arises when managers fail to set expectations or provide timely feedback, leading ...assistant. However, I recognized the need to shift my mindset to empower my team and allow myself to focus on...

... infecting non-dividing cells and so researchers set out to create a better, safer system. Unlike the ...vectors was developed for safe use in a clinical setting. In this generation, the HIV Tat gene (previously...generation. Knowing this you can scale up your production set-up and purify and concentrate your lentiviral particles...chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed together... my tip here is to test several protocols as the set-up depends on the type of cell you would like to ...

...projects and test novel approaches, a necessary skill set for any scientist. In this blog, we’ll delve into... few that I frequently observe in a scientific setting.  Powerlessness It’s hard to be creative when you...giving yourself a quick, easy goal. For example, set a timer for five minutes and come up with as many...processes often have to change with them. If you can, set aside time to prepare for future challenges. This...

... hour that morning scrambling. Everything we had set up involved multiple people in tight locations. Conference...in their chair not watching their actors live on set, but peering at a 7-inch wireless monitor. Wireless...needed to design a system that would allow me to set up a camera in a room, but still have control over...getting in close to mic them up. Monitor: I then set up a monitor connected to the wireless transmitter...Forward Design: We produced a video celebrating Massachusetts Nonprofit Day, which was the perfect opportunity...presentations and interviews. But how can I adapt this setup for the lab, so we can get back to producing protocol...videos?   Figure 4: The remote directing setup, complete with wireless video receiver, camera control...

... their practical brightness in your experimental set up. The practical brightness takes all application... conditions. By determining the brightness for a set of fluorescent proteins (with the same reference ...conditions (e.g. different emission filters) or different set-ups. Many thanks to our guest bloggers Joachim Goedhard...fold well or cannot be detected in a microscopy setup has zero practical brightness and is of no use. ...

...pathways as part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are the product...phenotypes. Since time is of the essence in clinical settings, looking ahead, we’re starting to build saturating... Phenotypic Characterization of a Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell...

...using computational approaches to analyze large data sets (myself included) and we are faced with finding ...keeping track of computation work comes with new sets of challenges. One challenge with computational ...similar mistakes in the future. Stay organized Set up a table of contents in the top directory: A directory...

...receptor research, and has generated a set of comprehensive datasets that collectively address many important...so coregulators, and a greatly expanded set of links to datasets and reagents in the field. Additional ...function as ligand-dependent sensors for a diverse set of fat-soluble hormones, vitamins, and dietary lipids...nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the expression patterns...seeking to compliment these consortium-generated datasets with a systematic effort to compile NR- and coregulator-related...coregulator-related global gene profiling datasets in to our data mining tool, Transcriptomine. We are...Additional information can be found in the Datasets and Reagents sections of the current NURSA website. NURSA and...

...light-up bacteria unwanted in food, healthcare settings, etc. What project in the Collins lab inspired...about finding the best product-market fit for this set of technologies, effectively communicating the advantages...must work well together, have complementary skill sets, and “talk a good game." Mike recommends that the...

...accountability that makes mentoring effective Goal setting is easier with more perspectives contributing to...process. Have each person write a Development Plan. Set concrete goals with input from the group to make ...practice meeting people – compete for fastest to set up a coffee date or most cards collected. The next...’s Association (HBA) Boston Chapter and the Massachusetts Association for Women in Science (AWIS) mentoring...

...species, with components quickly losing activity. I set out to collect the features that would define a good...useful. To apply these principles in a biological setting, we first need to define what features would allow...Click Chemistry, Click Biology can be defined as a set of reactions derived from the regular building-blocks...

...ended up at pgEd, what I do there, and how you can set yourself on a path towards a similar position. How...speak about our experiences in a variety of other settings, including “conventional” academic venues such... to take care of the organizational logistics. Setting yourself on the path to science policy and science...

...Harvard Medical School, and the Doudna Lab at Berkeley set out to decrease the off target effects of CRISPR/...,3). Stable strand separation is maintained by 2 sets of interactions : 1. Interactions between the non-target...off target effects in applied and/or therapeutic settings. Will combining the different mutations found ...

...searching and reading a regular part of your routine. Set a goal to read a certain number of papers each week...up with the literature in your field of interest. Set aside some time each day or week to at least scan...titles of what’s going on in your research area. Set a goal for paper-reading, even starting out at 1 ...

...limitations of current methodologies. This section sets the stage for why your funded proposal will address...advisors Once a rough draft is in presentable form, set up a meeting with your advisor to go over format,... apply for funding improves the associated skill sets. Increased experience in communicating and motivating...detailed scope of specific aims, milestones, possible setbacks, and potential alternative routes, Provides ...

...The moderator (possibly with an organizer) should set up a 30 minute call with each panelist to get to ...remind them about the event as it approaches. Room set-up and logistics To make a really lively discussion...level as the audience. In my experience the best set-up is panelists in a slight semi-circle (so they ...