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We narrowed to 74 results for: sgrna

Showing: 41 - 60 of 74 results

  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ... plate, co-deliver into the cells with the Cas9/sgRNAs vector (like PX330). For large changes (>100 ... a typical size (~5 kb) donor plasmid with Cas9/sgRNAs vector, for one well in 24-well plate. A good example... a typical size (~5 kb) donor plasmid with Cas9/sgRNAs vector, for a one well in a 24-well plate. A good...

  2. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...lab used single-AAV delivery of Nme2Cas9 and the sgRNA to demonstrate efficient editing in adult mice and...

  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...of 5%. Your experiment involves making a large sgRNA library to thousands of genes and targeting and ...sequencing millions of cells. For every individual sgRNA, you recover a minimum of 1,000 targeted cells to...

  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity...Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the immobilised ...

  5. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... expression. They replaced the tetraloop of the sgRNA scaffold with a com aptamer which binds an aptamer...

  7. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...regulator. The lab expressed both dCas9 and the ctrA sgRNA and saw that the levels of ctrA mRNA decreased after...

  9. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...many software tools predict off-target effects of sgRNAs using computational methods. They identify possible...mismatches based on the sequences of the genome and sgRNA. This is a good starting point for most researchers...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...is described in Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench...Doench & David Root and are described in Optimized sgRNA design to maximize activity and minimize off-target...

  12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...deposit please sign up here.  Human whole genome sgRNA iBAR library Article contributed by Susanna Bachle...Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu et al., 2019.  Wensheng Wei's...their loop region. After infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs...the gRNAs and their iBAR sequences. Since the sgRNAiBAR library covers every annotated human gene, it ...number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome Biology, 2019. PubMed...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ... predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:... Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a matching ...

  18. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016...

  19. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...specific RNAs, such as Cre mRNA or Cas9 mRNA and sgRNA, in human and mouse cells. They further develop ...
Showing: 41 - 60 of 74 results