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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 45 results for: sgrna vector

Showing: 41 - 45 of 45 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... we would recommend using a cDNA overexpression vector rather than going through all the troubleshooting... as WU-CRISPR (Wong et al., 2015)) or our lab’s sgRNA scorer 1.0 (Now 2.0) (Chari et al., 2015) and picking...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...The gRNA oligos are subsequently cloned into your vector of choice using the adapter sequences that you ...28414855. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target...Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for CRISPR screening....

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites or gateway compatible...neuronal tools to the expanding gateway compatible vectors currently shared amongst the zebrafish community...discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available at Addgene. ...for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here. Dimidschstein...Very low off-target activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low ...low off-target activity; mammalian expression vector Rees H, et al. Nature Communications. 2017. PubMed... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...depending on perspective – to work with inefficient sgRNAs in my initial direct screening efforts, which were...modular toolkits build single plasmid targeting vectors that encode the gRNA transcript and the repair ....114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection...
Showing: 41 - 45 of 45 results