Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 44 results for: stem loop

Showing: 41 - 44 of 44 results

  1. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...engineering in 2012.  Many of these advances have stemmed from the goal of reducing off-target Cas9 activity...Phillips-Cremins JE (2019) LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods...their CRISPR system. This common optogenetic technique can be employed in other split systems where light...HDR using split systems Later in 2015, the Sato lab unveiled a photoactivatable system to cleave a target...activation using dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light ...Gersbach’s lab at Duke University developed similar systems based on the light-inducible heterodimerizing cryptochrome...proteins. The goal of both groups was to create a system that would use light to turn on and off gene expression...

  2. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...regions are often reciprocal, forming a neuronal loop which is activated and strengthened during memory...neuronal communication. Viral vectors and the nervous system With the advent of viral vector technology, new...peripheral site of infection to the central nervous system where it replicates, spreads, causes neurotoxicity...WGA-Alexa Transsynaptic Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection versus Intradiaphragmatic...delivery to the central and peripheral nervous systems. Nat Neurosci 20(8), 1172–1179. PubMed PMID: 28671695...serotypes 1, 2, 5 and 8 in the rat nigrostriatal system. J Neurochem 109, 838–845. PubMed PMID: 19250335... retrograde tracer in the rat brain hippocampal system. Brain Res 152, 145-150. PubMed PMID: 79432. Schwab...

  3. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...Expression of this system is tunable via a rhamnose-inducible promoter. While this system was optimized for...developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for ...for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com aptamer which binds... This editor still retains A-to-I editing. This system is called RNA Editing for Specific C-to-U Exchange...

  4. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...cholerae that encodes a Type I-F CRISPR-Cas system. This system has four major components: 1) a CRISPR RNA...eukaryotic genomes.  Components of a transposon system Transposon: The transposon is the DNA sequence...take a closer look at three different transposon systems adapted for use as research tools: Sleeping beauty...otherwise, piggyBac was discovered in the cabbage looper moth (Potter et al., 1976). Its target site is ...ve learned about some of the popular transposon systems, let’s take a look at how they can be used in the...Transposable Elements by Guide RNA-Assisted Targeting) system. INTEGRATE is based on a naturally occurring Tn7...transposon end sequences (Fig. 3A).  For the INTEGRATE system to transpose, first the QCascade complex surveys...
Showing: 41 - 44 of 44 results