...CMV-driven; CROPseq) Mammalian AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_...Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown...Sontheimer, E. J., Goodale, A., Greene, M. A., Green, T. M., & Doench, J. G. (2024). Modular vector assembly...

...glycoprotein along with the TVA receptor in neurons using AAV and then delivering rabies dG pseudotyped with EnvA...example, LDLR expression in hematopoietic stem cells, T cells, and B cells is low. Infection of these cells...Liong SH, Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo...nmeth999 Wickersham IR, Lyon DC, Barnard RJO, Mori T, Finke S, Conzelmann K-K, Young JAT, Callaway EM (...

...plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming the surface..., F., Nalin, R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence.... L., Veseli, I., Moore, R. M., Kiefl, E., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B.,...

...systems have enzymes small enough to fit into a dual AAV system, albeit with lower knockdown efficiencies....HITI and HDR. PASTE is also compatible with both AAV and adenovirus templates. The system, which is larger... 6. PMID: 34489594. Eleonora I. Ioannidi, Matthew T. N. Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski,...

...elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing...across four different types of human cells, including T cells and hematopoietic stem and progenitor cells....

... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...hard-to-study genes in hard-to-study cell types. Natsume T, et al. Cell Rep. 2016. PubMed PMID: 27052166 Nishimura...containing ten guides against each of the 8,158 predicted T. gondii protein-coding genes). Dr. Lourido’s studies...are conserved among apicomplexans and important for T. gondii fitness. Sixteen of these genes were investigated...was shown to be important for the initiation of the T. gondii infection, and necessary for the asexual cycle...

...lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments...with a single viral infection. References Maetzig T, et al. Gammaretroviral Vectors: Biology, Technology...

...effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express...10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled...

...resources on the Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...your experimental values and your controls. ANOVA, t-test, multiple regression analysis, and non-parametric...Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer CT... https://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA...Thelwell N; Millington S; Solinas A; Booth J; Brown T. Mode of action and application of Scorpion primers...

...Acids Delivery, or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9.../doi.org/10.1002/0471142905.hg1508s88 Leidy-Davis T, Cheng K, Goodwin LO, Morgan JL, Juan WC, Roca X, ...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin...

...acids) means they can be delivered in vivo using AAV vectors. Having a way to shut off CRISPR-Cas systems....2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber A (2018) HDAd5/35++ Adenovirus Vector Expressing...

...Resources on the Addgene blog   Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview .... S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, M., Sim, B., Mustafa, M., Nik...

...targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase.../10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, Snowball A, Knauss...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern CH, ...

...AdVs have already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the... M, Maggio I, Henriques SFD, Janssen JM, Cathomen T, Gonçalves MAFV (2014) Adenoviral vector DNA for accurate...

...to their transduction has been shown to increase AAV transduction efficiency by approximately 15-fold ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias...

...QCascade’s genomic target site.  T-RL= transposon right-left orientation. T-LR= transposons left-right orientation... advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, ...

...respectively, to obtain PEs small enough to deliver with AAVs but still efficient at long and complex edits. The...Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M., Mercer,...McCreary, J., Pierce, S. E., Tolar, J., Meissner, T. B., Chaikof, E. L., Osborn, M. J., & Liu, D. R. (...://doi.org/10.1038/s41586-024-07259-6 Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, ...

...Thy1-YFP-H mice).  However, we recently developed AAV (adeno-associated virus)-based fluorescent labeling...Pubmed. A. Ertürk, C.P. Mauch, F. Hellal, F. Förstner, T. Keck,  K. Becker, et al. (2011). Nature Medicine,...cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter...

...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo...Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024...

.../10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, Zhao T-T, Connelly JP, Pruett-Miller SM, Blanc V, Davidson...iSRIRACCHA) where Cas9 expression is activated using 4-OH-T and doxycycline. A gRNA that targets your desired ...editing at three different genomic sites in the AAVS1 locus using GEmCherry2. In this experiment they ...