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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 93 results for: tac promoter

Showing: 41 - 60 of 93 results

  1. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... vector is inserted downstream of a functional promoter (gene traps). The resultant gene targeting system...pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR gene. To modify these tagging vectors...pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances...Central PMCID: PMC2577350. 4. Kim, Jung-Sik, et al. "Intact cohesion, anaphase, and chromosome segregation ...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...transcription by placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores ... RNA Polymerase III. By expressing Corn under promoters for transcripts uniquely transcribed by RNA Polymerase...with DFHBI, but its structure allows for greater contact with its RNA aptamer. This increased interaction...

  4. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences of the gene... available at Addgene, also gives the option to stack several expression cassettes onto a single T-DNA...

  5. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...Blugene needs to be friendly, personable, fun, and promote our values of community and sharing.  One big feature... inspired to tackle your next science challenge. If you have any feedback, please contact us and let us...

  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...the database by certain features like serotype, promoter, or tool type and each entry contains an image... data or lab notebook handy?  Contact info: Give us your contact information so we can follow up with...

  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... and partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or... in epitope tags? Others may be interested in attaching epitope tags to their genes of interest, allowing...

  8. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...connecting patients to important resources and promoting research for rare disease cures. Through their...animals models of the disease in partnership with Taconic Biosciences. The study of peritoneal mesothelioma...

  9. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ... Kamens, Addgene's Executive Director, "and to promote the great work of Michael Davidson and his colleagues...variety.” In most cases, backbones are available to attach the gene of interest to either the N-or the C-terminus...

  10. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...one of the most common criteria required for a promotion. Yet little explanation is given for how someone...the work gets done Managers do this by removing obstacles that stand in the way of the doers and by ensuring...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...You will have the option to indicate separate promoters, tags, or cloning information for each insert.... 8: Add vector backbone information. List any promoters, cloning details for how your gene was inserted...greater than 75 plasmids, please let us know by contacting [email protected]. These deposits are considered...us at  [email protected] Special cases Please contact us at [email protected] or +1 (617)-ADDGENE (...deposit a pooled DNA library with Addgene?" or contact us at [email protected] or +1 (617)-ADDGENE (...genomic alterations unique to your strain. Please contact us at [email protected] or +1 (617)-ADDGENE (...

  12. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet aggregation, and ...Research tackling questions in the fields of Cancer, Inflammation and Immunity, as well as various combinations...Researchers and Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary.... to understand cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few...are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis The conference...d like to share with the scientific community, contact [email protected] to find out more about our ...

  13. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...of these three enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator... to study these mutants.   Fig. 2: (A) Betacyanin is produced from tyrosine by the activity of ...

  14. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...released into the environment. Various factors promote natural transformation in different bacteria such... or sex pilus that attaches to the recipient cell drawing it close. Once in contact the donor can transfer...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...bacterium to a recipient bacterium through direct contact. The donor bacterium contains a DNA sequence called...

  15. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ... and offer congratulations on job changes and promotions.  Follow academic scientists on ResearchGate ...would like to try and meet. Don’t worry about contacting someone much more senior than you – meeting scientists... level of training is always a good idea. Make contact with other attendees in advance. Join a LinkedIn...

  16. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...straightforward to define, but for control elements such as promoters and transcription terminators, the boundaries ... common feature detection in a new way, please contact us at snapgene.com. Many thanks to our guest bloggers...

  18. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...was shown that putting the operon under a strong promoter results in high R-body expression  (Schrallhammer...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

  19. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...will be suitable). For N-terminus tagging, the promoter region of both the alleles should be sequence-...cautiously analyzed for any damage (indels) to the promoter region of the non-HDR allele. For N-terminal tagging... if you have any questions, do not hesitate to contact me at [email protected]. Keep pipetting and...

  20. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized...the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at a time Since...
Showing: 41 - 60 of 93 results