We narrowed to 95 results for: tet on

.... coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9...genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed...

...complexes with LacZ𝜔 and forms an active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside...

...DNA-binding domains, including the commonly used LexA and rTetR systems such that they activate gene expression ...advantages. T-DOGGs can be made drug-inducible using the rTetR system, but Cre-DOG lacks the toxicity that can ...

...speed of labeling. BioID requires 6-24 hours for tetracycline induced expression of the BirA* fusion protein...Split-BioID, Schopp et al allowed 24 hours for tetracycline induced expression and biotinylation of interacting...

...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance axonal labeling and synaptophysin-tagged...

...elegans embryos. However, this initial timer was a tetramer with a propensity to aggregate in cells (Tsuboi...have realized how problematic aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper...

...interactions that uses split fluorescent proteins tethered to potential protein interaction partners. Unlike... form the complete FP because the PIPs they’re tethered to bring them into close proximity (Figure 1)....

... fluorescent protein were fused in-frame to a tethering partner, which directed localization of the fluorophore...Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm...

...while nuclease-deactivated Cas9 (dCas9) can be tethered to a repressor (CRISPRi) or an activation domain...RNA-guided DNA binding protein (dCas9). dCas9 is then tethered to a repressive domain, such as KRAB, to prevent...

...antibody against a target protein is immobilized, or tethered, on agarose or magnetic beads and then incubated...antibody binds to the target protein, thereby tethering it to the beads. Unbound proteins in the mix are...

... different induction systems, such as PT7, Prha, Ptet, and Para. The pDonor plasmid pRE57-Ter with multiple... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

...on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker KanR....

...BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901 gene downstream...

...system is to use a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red...

...CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming to ...CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest in using...

...CRISPR/Cas9 compared to ZFNs and TALENs,” said Tetsushi Sakuma of Hiroshima University. “However, there...

...new feature in action, click here to explore the TetO-FUW-OKSM plasmid page. Or search for your favorite...

...researchers led by Dr. Jorge Ruas of the Karolinska Institutet, the discovery really wasn’t what they’d expected...

...chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally abundant. Second...

...ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com aptamer which...