...homepage is still important. It needs to express value, clearly communicate exactly what a site offers ...and navigation, we also wanted to emphasize the values that make Addgene unique: community, sharing, and... easy and fun every day.  With these three main values we set out to radically redesign our homepage to... and made a commitment to reinforcing the three values with each element. We also set out to make sure...to be friendly, personable, fun, and promote our values of community and sharing.  One big feature upgrade...

... for validation data on IBEX reagents.  mAb3D atlas validated antibodies: A database of validated antibodies...These links can help you find experimental and/or validation data on molecular biology tools.  Addgene Data...Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors.  Caspedia: A data repository...

...immobilization for kinetic analysis Proteolytic removal of the fusion protein could provide a more relevant...second immobilization method. One possibility is covalent modification of the purified target protein with...and neutravidin) is for all practical purposes covalent. Therefore, protein immobilization through biotin-avidin...amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids. Unfortunately... the erythropoietin receptor and subsequently evaluated interactions with mutant and wild-type erythropoietin...

...reagents If you want to label intracellular proteins covalently with FRET acceptors, then biarsenicals are a ...The utility of these reagents is that binding is covalent, so free dye can be washed away. In addition, ... define the maximum and minimum energy transfer values you can expect, thereby establishing the dynamic...design lends itself to creativity and innovation, valuable commodities in any NIH grant application! Using...Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling calibrated FRET distance measurements...Stephen R. Adams, and Roger Y. Tsien. "Specific covalent labeling of recombinant protein molecules inside...

... administration, and dose (Lu et.al., 2020).  Evaluating a drug’s immunogenicity in-vitro helps researchers...in vitro assessment of immunogenicity can help evaluate risk of an antibody.  In silico prediction of...EpiMatrix is a commercially available algorithm to evaluate the immunogenicity of therapeutics in silico. ...of in vitro prediction of immunogenicity is to validate the findings from the in silico assessments, as...prediction algorithms fail to capture. In vitro evaluation of ‌therapeutic immunogenicity is typically performed...

... best to either select antibodies validated for ChIP or to validate your antibody on crosslinked proteins...for exploring these interactions, and can give valuable insight into DNA expression and protein interactions...for the first time, look for one that has been validated in multiple assay types. Remember that ChIP uses...uses native proteins (so antibodies validated only for denatured proteins may not be a good choice) and...

...completely missing in women. Bicuspid aortic valve (an aortic valve that has two leaflets, instead of three...murine solute channels, the team will be able to validate their findings in mammalian cells with both human... are two heart defects that are 50 times more prevalent in women with TS. “The goal of our study is to...understand why women with TS develop bicuspid aortic valve at a rate that is 50-100 times higher than women...

...performed during your harvest. Validation Recombinant antibodies should be validated to ensure they bind their...antigens specifically. Possible applications for validation include western blot, immunohistochemistry, and...produced, harvested, purified, quantified, and validated, it’s ready for use! You can learn more about ...coefficients of monoclonal antibodies with experimental values as measured by the Edelhoch method. Int J Biol ...

...are critical to stem cell survival. Knowing which genes influence the survival of our cell culture is the...promoters, insulators, and more. I then identified valid gRNA sequences without off-target effects for the...CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome Edit Resources on Addgene.org Find...Your Research Check out Our CRISPR Guide Find Validated gRNAs ...

... sgRNA Addgene may carry a validated sgRNA for your target gene.  Validated gRNAs are a great bang for... freely available sgRNA design programs. Find Validated gRNAs for Your Next CRISPR Experiment Cas9 expression...Mammalian Expression Systems and Delivery Methods”. Validating your edit Once you have delivered Cas9 and a ... found in our blog post entitled “CRISPR 101: Validating Your Genome Edit”. Is Cas9 not your Cas of choice...

...infected with Cas9. Validating the Library The CRISPR knockout library was validated via a growth screen...are used to call hits from the growth screen, p-values were less significant than when non-targeting guides...guides are used as controls. Less significant p-values lead to more false-negatives results (i.e. failing...library resulted in both increased and decreased p-values of known ricin regulation genes compared to results...

...is a values-driven nonprofit. Recent remarks and actions by X’s leadership conflict with our values. For...monitoring, and resharing content on X.  Addgene deeply values our community and recognizes the impact of this...allows us to remain aligned to our organizational values.  To preserve the knowledge, conversations, and...

...we've already taken them for a test drive! We validated viral packaging and strong expression after inserting...characterization at IPI as well as community-led evaluation, and the results are available to view from the... of GABAergic interneurons — those marked by parvalbumin (PV), somatostatin (SST), vasoactive intestinal...neurons. The authors characterized these tools and validated their specificity in mice, non-human primates,...under the control of the BiPVe3 enhancer and parvalbumin (PV) immunostaining (green), in the primary somatosensory...

...virus, a successful AAV experiment starts with validation and optimization. Although there are different...find it is usually faster and easier to do the validation first.  AAV-mediated gene expression can differ...inducing drug, so be sure to test for this when validating your viral vector. Titer Once you have selected...measurements. The number itself is determined through validation and optimization of your expression system for...don’t. The key to good AAV selection …is truly validation and optimization! Understanding serotypes, promoters...

...of DNA transposon transposition. Modified from Sandoval-Villegas et al., 2021. To mobilize a transposon...inactivated Tc1/mariner transposons found in fish (Sandoval-Villegas et al., 2021). Sleeping Beauty’s preferred...or negative selected for during the experiment (Sandoval-Villegas et al., 2021). Target site preference... including zebrafish, mice, rats, and rabbits (Sandoval-Villegas et al., 2021).  Gene transfer Transposons...topicpage/transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021...

...mycoplasma removal from the infected culture. MRAs are non-toxic, effective in the removal of most of ...Started with Tissue Culture below! Mycoplasma removal after detection So you found mycoplasma contamination...contamination to other healthy cell lines. Mycoplasma Removal Agents (MRA) are derivatives of the quinolone family...mycoplasma. These methods cannot guarantee complete removal of mycoplasma but, can get rid of most of mycoplasma...

...Preserving scientific assets: We hold a repository of valuable research materials that might otherwise be lost...and verifying them independently — particularly valuable when budgets are constrained. This collaborative...limited resources. These resources become even more valuable as labs look to maximize productivity and efficiency...impacting your work and what support would be most valuable from Addgene during this time. Your input will...

...University Cancer is one of the greatest examples of survival of the fittest. Cancer cells find a way to grow...from Madame Curie), chemotherapy, and surgical removal of the tumor. However, these conventional therapies...the immune system from fighting cancer. Surgical removal causes increases in tumor cytokines like TGFβ and...immunotherapy target using diagnostic tests and biomarker validation to identify populations that are the most likely... fosters immune privilege and predicts reduced survival." Nature medicine 10.9 (2004): 942-949. PubMed...

...important, targeting all genes doesn’t necessarily add value if you are addressing a well-defined, hypothesis...druggable targets, preferentially those for which validated inhibitors or even approved drugs already exist...smaller, secondary, CRISPR screen could be used to validate the list of candidate genes. Green Listed, as ...and then use Green Listed to design a smaller validation screen using selected gRNAs extracted from the...an easily measureable cellular phenotype (e.g. survival of a cancer cell as a cytotoxic drug is added)...

...Data Hub, an open-access repository for antibody validation data. We’ll continue growing our educational ...seizing new opportunities to create even greater value for the scientific community. As the executive director...director of Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work. In 2024,...throughout 2024 on Addgene’s work, mission, and values. I welcome feedback and commentary from our diverse...