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We narrowed to 397 results for: ARA-2

Showing: 291 - 300 of 397 results
  1. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...PMID: 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...school classrooms, like gel electrophoresis to separate DNA or transforming cells with green fluorescent...in functional genomics research Learn how the Arabidopsis research center brings plant science to life ...
  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ... 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3, CHO, 293HEK...called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction... a problem depending on the duration or other parameters of your experiment. Mammalian cells double at...reduces the rate of plasmid dilution but does not guarantee 100% transfection efficiency. Stable transfection...
  3. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...create a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within...the antigen-binding sites of the antibody - the paratopes - may change and impact the specificity and avidity..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P...Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR...
  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ..., N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific DNA recombination in mammalian cells... it, allowing for two separate recombination events to be controlled in parallel. Have you used the Cre-lox...consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric core spacer sequence... which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a...forming a dimer. The two loxP sites then align in a parallel orientation, allowing the four Cre proteins to...maintained).  Translocation: If the sites are on separate DNA molecules, a translocation event is generated...Cre into cells of interest. What if I need two separate recombination events? One potential limitation...
  5. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...affinity and specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 ...kill the target cell. If the pathogen is a large parasite, ADCC can be induced through a very similar interaction...
  6. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...easily understood and consistent in design and layout. 2. Be concise in your writing  When writing a manuscript...worthless. But simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts...
  7. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...the world’s population, we account for an estimated 2% of global plastic generation. Less than 9% of plastics... References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender Law. "Production, use, and fate of all ...
Showing: 291 - 300 of 397 results