We narrowed to 904 results for: CAN

...which can “drag and drop” kilobases’ worth of DNA with CRISPR-driven precision into a genome, can be considered...collateral" activity of the enzyme can lead to off targets and result in significant, negative effects in RNA targeting...forward for what the field can do.    References and Resources References  Özcan A, Krajeski R, Ioannidi...tests samples of new bacteria from anywhere they can find, whether the samples are from a rain gutter ... instance, a disease like cystic fibrosis, which can be caused by thousands of different mutations, all...lab switched up the serine integrase for one which can enzymatically take a genetic sequence and integrate...cargo via Cas9-directed integrases.   Use PASTE can be delivered via a single dose of plasmids, works...

...issues as a region of interest can be targeted using a gRNA, which can easily be cloned into a gRNA expression... as dCas9 (Jinek et al., 2012). Like Cas9, dCas9 can bind to a specific DNA sequence via a targeting gRNA...DNA-binding molecule, such as Zinc-finger or TAL proteins, can be used. The Fujii lab demonstrated this in mammalian...proteins targeting a specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013...identification of DNA binding proteins, this method can characterize nucleic acid-nucleic acid interactions...successfully used in this "in vitro" form. Any tag system can be used to isolate genomic regions of interest with...biotin ligase. Biotinylated dCas9, with bound DNA, can then be purified by streptavidin high-affinity purification...

...two additional parameters that can affect FRET efficiency. These issues can be overcome by choosing a complementary...coupling." The emission of the acceptor fluorophore can be measured using microscopy techniques. FRET measurement...fluorophores and their orientation to one another can affect FRET. When it comes to studying an unknown...measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent fusion...fused between ECFP and Venus Other issues that can affect FRET measurements include: the brightness ...pair too close to each other in the spectrum, you can easily directly excite the acceptor with the laser...the FRET methodology for a given experiment. You can then choose the most efficient approach for your ...

...hosted new posts on topics ranging from new tools you can use to monitor cellular perturbations to how CRISPR...These properties, combined with other benefits you can find in the post, may make Cpf1 the preferred CRISPR...editing this post, I learned a lot about how FRET can be used to study biological phenomena and about how...how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments. Professor...recommend this post to anyone wondering if and how they can apply FRET in their own research. All posts from ...

...guidelines to follow in order to ensure that people can quickly and easily identify what your plasmid contains...your plasmid name. This simple piece of information can often convey many important details. Once you know...know the backbone a plasmid is based on, you can usually derive: a) the bacterial antibiotic resistance...scientist to know what is in a plasmid and often how it can be used just by reading the name. For more information...information about plasmid features and tips you can use in the lab, check out more posts in our Plasmids 101...

...Indeed, this method can be used at high throughput because numerous cell lines can be generated, each ...the pull-down assay, wherein a protein of interest can be captured along with its associated partners. Common...biochemical “epitope tag” such that these tagged proteins can be immunoprecipitated using a standard tag-specific...interest, and a universal all-purpose “tag antibody” can be used for downstream IP/ChIP assays. Creating ...epitope tagging. Using our 96-format pipeline, we can routinely achieve 5-30% tag knock-in efficiency across...Integrated DNA Technologies, USA). These synthetic RNAs can also be purchased from various other suppliers with... it is also straightforward as the RNA molecules can be directly purchased and there is no need to construct...

...(Martella et al., 2017). If you can dream up an experiment, MoClo can help you achieve it! More recently...constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free expression systems...IIS restriction enzymes (Weber et al., 2011). It can be performed at multiple levels to generate anything...variety of purposes beyond what we discuss. If you can think it, MoClo might help you achieve it! MoClo...The EcoFlex Kit has a wide array of DNA parts that can be assembled into individual TUs or very large (up...offers a one-pot reaction wherein multi-part assembly can occur. It is quite extensive with a supplemental ...special attention to protein modifications which can be especially difficult in alternative expression...

... proximity of the two proteins can be inferred. Intermolecular FRET can be experimentally difficult to...push these tools even further? All of these things can be done using FRET! Read on to find out more about...dipole-dipole interaction. The efficiency of FRET can be measured for a given donor-acceptor pair and a...consideration for a FRET experiment, the possibilities can seem overwhelming given the large assortment of fluorescent...proteins are available on our FRET Resource page and can be used to create fusions with a gene of interest...efficiency and any unpaired fluorescent proteins can contribute additional noise to the measurement. If...FRET, well-characterized FRET reference standards can be used to validate FRET measurements and serve as...

...extrachromosomal arrays can lead to artifacts due to overexpression. CRISPR can insert the FP gene into...they are great in number, generally very small, and can contain different neurotransmitters. Using genetically... synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity without... large genetic changes in C. elegans with CRISPR can be difficult since it requires the much less efficient...single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR to introduce large FP genes into C...mouse embryonic fibroblasts. This fusion protein can be used to determine cell cycle length by examining...Addgene CasRx is a RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids...

...an initial reversal drive cannot restore the exact original sequence, it can restore the original protein-coding...distort inheritance in their favor and consequently can spread even when costly. If even a single organism...appropriate flanking homology into a germline cell can create an RNA-guided gene drive. In principle, this...combination with standard barrier protocols, they can reduce the risk of accidental escape to a negligible...Molecular confinement involves building gene drives that can spread through populations of transgenic laboratory...to human error. And with gene drives, one mistake can be enough. Reversal drives are designed to overwrite...sequence using a recoding strategy; a subsequent drive can restore the wild-type sequence (save for the residual...