...question to ask is what to do with them. In some cases, there might be a mutation that disrupts the function...mutation in the same genetic background. In other cases, YFG or one of its mutant variants will need to ...systems exist (eg. LexA/LexAop and Cre/Lox systems) in case you want to express one gene in one cell type and...

...FAP - fluorogen activating protein - dL5** in this case), and 2) a fluorogen (the TAP - targeted and activated...activated photosensitizer - MG-2I in this case). The fluorogen is not fluorescent until it binds its protein...easy to watch development in real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization...

...unmeasurable skills and productivity coming from that casual discussion on the way to the office or in the kitchen... flexible work schedule the more we realize how casual chatter is very much missed in the virtual world...widely used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas....

...depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive... Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells ...

...analysis performed in experiment 1. Use camelCase: In camelCase, each subsequent word in a file name after... more than one word should be named in the camelCaseFormat since spaces between each word can make it ...

...in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering (Ref...using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering of the.... "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643 (2017...

...will be blocked during protein folding, in which case you should try tagging the opposite end.  If neither...disrupted by denaturants (Hochuli, 1988). In some cases, proteins can be bound under denaturing conditions... aren’t any good antibodies available. In these cases, having a tagged version of the protein is hugely...

...entirely, and our software needs to handle these cases. The following code examples show how software can...sample and get it ready for shipping. In the first case, we naively assume that everything will go perfectly...we can interchange them easily as needed. In this case, the physical considerations of the laboratory make...

...' decisions to alter postdoc salaries - in some cases reversing these decisions entirely. At Future of...or are being compelled at the state level, in the case of some public institutions) to cancel plans to ...communication of their plans (many postdocs - and in some cases, faculty - have found out about what their institution...

...cytometry, secondary antibodies may be indicated on occasion, usually after other signal amplification techniques...indiscriminately, giving them the same output signal. In these cases, it is best to use the direct method when possible...confidently, collect a broader array of data, and occasionally ask themselves and others that most perplexing...