...Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition... always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback. Happy...

...number of CRISPR libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection of CRISPR ...As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on...community. They did this by depositing them in Addgene’s repository. Other researchers, eager to try this... tools of their own to share. Over the years, Addgene’s repository has grown to include many CRISPR tools...This growth is supported by new labs joining the Addgene CRISPR community, with approximately 100 new CRISPR...CRISPR depositors each year.    Figure 1: Addgene CRISPR distribution and deposits. (A) Cumulative... (B) Cumulative CRISPR plasmids deposited with Addgene by year. (C) Cumulative number of laboratories ...

...Communications Manager Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a ...still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product... of Addgene’s culture. Have you read any of these books? Whether you have or not, let the Addgene community...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...and explains the origins of ABC: I founded the Addgene Book Club (aka ABC) in 2011 after having numerous... activities at Addgene ...

... researchers with many different ways to control gene expression. Get the full scoop from Addgenie A. ...how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6. CRISPR 101: Non-homologous...Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have limited packaging capacity making...2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...have started providing recorded interviews with Addgene community members. In addition we’ve experienced...them a read if you’ve missed them! Breakdown of Addgene's top blog posts from 2015 Our most popular posts...your own guest posts by e-mailing us at blog@addgene.org. Thank you again for a fantastic year and happy...

...is in this subject.” Outreach travels with Blugene Blugene loves to visit researchers, so Outreach Scientist...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from...their day with breakfast and an introduction to Addgene with our Executive Director, Joanne Kamens. As ...to start networking! Learning about careers at Addgene Afterwards, the students split into two groups ...groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology...slideshow from our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how...with the “comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty...

...this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation should...and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species...Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics: Introduction and Opsin Delivery The...considerations you should think about when developing optogenetics behavioral experiments. There are far too many...many behaviors that have utilized optogenetics to be fully summarized in a short blog post, but some examples...ill-defined region of the brain. With the advent of optogenetics, ICSS can now be done with neuronal cell-type...the literature to find all the applications of optogenetics relevant to your field. Ultimately, your question...

... development of optogenetics, has genetically manipulated a number of the opsin genes to alter the light...have also been engineered to express various opsin genes [7-9]. The advantage of using these lines is, if...Regardless of the method of expression of your opsin gene, it is essential to test the expression of your ...PMID: 22470147.  6. Gore BB, et al. Manipulating gene expression in projection-specific neuronal populations...sections of optogenetics papers. Optogenetics step one: Deciding how you want to use optogenetics The most...things in science, optogenetics is best used to address a specific question. Optogenetics is a tool and, ...electrophysiology to prove that your optogenetic manipulations are generating action potentials (or lack there...

...Hey guys, Blugene here! As Addgene’s mascot, I’ve watched Addgene grow into the successful, internationally...to the entire Addgene family; the depositors and requesting scientists, all of the Addgene employees, the... the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped Addgene grow and...changes at Addgene over the last 10 years as well as the many people that make up the Addgene family. It...Ken Fan each spoke about what Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination...make copies of the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community...

...where his group focuses on gene regulation in nematode molting and spermatogenesis. His group also builds ... one of the model organisms which I use to study gene regulation during developmental processes, CRISPR...Craig Mello’s lab – used inactivation of the unc-22 gene as their selection marker (Kim et al., 2014). Meanwhile... I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency (0.13%...yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa), visualize specific...Gaul U, Forstemann K (2014) Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous...CRISPR toolbox for C. elegans: Cpf1 as an alternative gene editing system for AT-rich sequences https://doi.org...

...ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering ...reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition of standards...antibiotic resistance markers, promoters, and inducible gene expression systems in bee gut microbiome species...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...address these questions, biologists often use next-generation sequencing. Sequencing the 16S rRNA reveals the... also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take a look ...microbiome with sequencing is vast, the ability to genetically alter microbiome members is limited and many ...