We narrowed to 798 results for: KIN

...,” said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate the...For more information on choosing a backbone and making fusions, see this blog post from Joachim Goedhart...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many... many more! Baird has a helpful tip for anyone looking to peruse the collection without getting overwhelmed...

...paper they’re reading has been linked in a ReFigure making it easy for others to discover your insights on...researcher Christa Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure...PubMed Central, eLife, PLOS and Figshare but we are working on expanding our reach. We can’t save you the hard...career researcher and cofounded ReFigure with Joe Akin. She envisions a world where the impact of teaching...

...faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow...set of tools therefore depends on what you are looking to study. Figure 1: A schematic showing the...receptors in the brain. One way to address this is by working with organisms with light accessible brains, such...

...your plasmids are distributed. Next month, we are making two changes to the policy that will make the process...the plasmids online for distribution instead of risking leaving them on hold indefinitely. Of course, the...citation and release the hold. Increasing impact Making the plasmids available as soon as your paper is... paper comes out later. Or, if a project isn’t working out as planned, but you still hope somebody will...

...and you are smart, you can be a good manager. The kind of smarts and the type of skills that it takes to... from being included in organizational decision-making. It is imperative that employees are thanked for...manager keeps these factors in mind at all times when making decisions, assignments and polices. Sometimes the...priorities for advancement in science Have their own working schedules and may not necessarily be comfortable...

...persuing milestones and working hard. Being patient, being selective, and talking to peers and mentors will...with the current technology to identify a problem, asking what approach will I take and what can I learn,... Dr. George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists...

...inclination to be a teacher was looking for an outlet. I started asking people: why do you do what you ...bioscience into context, while at the same time working through the details of experimental design and ...of science communication professionals who are thinking about, for example, how to use new technologies...

...features provide extra layers of risk management when working with viral vectors. Typically, one day after transfection...particles are secreted into the cellular media, making viral collection fairly easy. Producer cell media...transfect’ cells lines are amenable to transduction, making it a logical choice for efficiency. Where viruses...with alternative methods. Ready, set, transduce! Making virus can sound daunting, but it is easier than...

...immunohistochemistry images that are crisp, bright, and lacking in non-specific staining can be a challenge in ... mixing your primary and secondary antibodies, blocking excess secondary with serum from the same species...problem.  Pre-block with Fab fragments (Figure 2C) Speaking of Fab fragments… Another strategy for species-on-species...0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

...just on the disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they...proteins. This is, for the intrepid researcher looking for molecular biology tools, a very useful trait...Created using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology...reproduce only in experimentally controlled conditions, making them far safer to work with than virions. The four...