We narrowed to 432 results for: sequence

...examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved sgRNA activity...Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA domains that...

...circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that...of proteins that are highly similar in terms of sequence and thus very difficult to distinguish using standard...

...cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells that stably express the dCas9-Suntag...cells were then isolated and their genomic DNA sequenced. Guide RNAs targeting several known and some unknown...

... and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional and lab...to the associated data (spreadsheets, images, sequencing data, etc.) used and/or referenced in the experiment...

...fluorescent signal. As for linker sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have ...

...positive or negative selection). Next-generation sequencing (NGS) to identify genes of interest. ...Kampmann, M. (2023). CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin...

...Droplets that fluoresce contain the amplified target sequence. After all of the droplets have been read, the...reason, it can be used in the detection of rare sequences and single cell analysis. It is also being used...

... still able to bind to the upstream activation sequence (UAS) of its target gene, in this case a reporter...downstream of a promoter containing the target sequence of the DBD. The DBD-POI fusion (commonly referred...

...However, iChIP requires insertion of recognition sequences of an exogenous DNA-binding protein such as LexA...to suppress PCR amplification of specific DNA sequences. Thus, we've made some contributions in technology...

...is quality controlled through next generation sequencing and spectrophotometry to test purity (260/280...