Skip to main content
Addgene
Showing: 581 - 600 of 779 results

  2. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...depending on the size of bacterial culture you’re working with. Overall, the glass syringe filter method ...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...the transformation efficiency of the strain: when asking the cell to perform multiple recombination events...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

  5. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... insert-specific sequence. This is a “yes or no” kind of test, with a positive clone amplifying a product...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...and public health and medical organizations are working to help educate the public about proper antibiotic...observe any mutations at the target site. In a mouse skin colonization model, phagemid treatment decreased...

  7. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...475nm Lyn-tag (derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human ...Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological...

  8. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp is a semisynthetic...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...library screen only to find the system itself is not working because your reporter protein doesn’t function ...Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid system. Nature Methods...

  11. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...and develop an area of expertise. Whether you’re seeking a full-time job or want to be an independent grant...a mentor, find your people Find someone who is working or has worked as a Grant Professional to mentor...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids ...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be...

  13. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...many questions from scientists all over the world looking for some additional information or clarification...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...the yeast extracellular surface) which enables tracking of protease activity with a fluorescent protein...that the best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., ... Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was first identified in 2016 by the...

  17. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...clear plans to connect Genotype to Phenotype thus making progress on the NSF “G to P grand challenge.” Creating...like people. This is actually rare in the animal kingdom. Gasterosteus aculeates (Dan Bolnick) A paper on...

  18. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...research vision uninterrupted and then you will begin working through your aims over the next 45 minutes. The...salient details. Make the most of your limited speaking time. Beyond the information you seek to convey...

  19. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...tools for cellular targeting. Lately, they’ve been working on two tools that expand and exploit CRISPR, both...Jonathan and experts in CRISPR evolution, were also working on the project. Using computational observations...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting...overly confluent or too old. Ensuring that you are working with healthy, happy cells is one of the easiest...
Showing: 581 - 600 of 779 results