We narrowed to 904 results for: ANT

...expression and largely defines the amount of recombinant protein that will be produced. Many commonly-...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...the first 24 hours of culture, when glucose is abundant and ethanol concentrations are low, pADH1 displays...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ... transcription from the UAS promoter. This is important because it allows them to be used together for...

...enable sorting and selection of transduced cells. Grant Challen’s lab also created constitutive (pCMV-dCas9...fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility, and this...recruit epigenetic machinery - but is there an advantage to using direct epigenetic modifiers? As with ...will determine the tool that you should use. If you want to study the effects of one particular modification...tool would be your best bet. Another potential advantage of CRISPR epigenetic tools is their persistence...

...Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for... to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh and Jonathan...your own work!) If you've got a research story you want to share on our blog, please reach out to [email protected]...

...introduction of the model antigen, Ovalbumin, into cell lines to study antigen-specific responses. Find...screens have been used to interrogate T cells, an important immune cell type, in more complex and physiological...for a screen that assessed genes that regulate antigen-specific CD8+ T cell responses to a murine tumor...

...can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...see from the community is your negative data. We want to make sure people are selecting AAVs that will...work was cited by others?)  Ready to submit?  Fantastic! The next 25 submissions will receive a 20% off...

...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...challenges. However, these same researchers face significant barriers to scientific discovery and career development...accelerate biology research in the labs they’re granted to, as well as shine a light on some of the great...

...identify what your plasmid contains and other important information. Tip: A lowercase "p" is often used...simple piece of information can often convey many important details. Once you know the backbone a plasmid ... on, you can usually derive: a) the bacterial antibiotic resistance, b) the promoter that drives the insert...position 295 was mutated to an Alanine, Q295A.  Mutant version: pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype...

... do not express Cre or Flp. Here at Addgene we quantify the amount of transgene recombination for every...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially...Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...

...component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended... Genome Portal at the University of California, Santa Cruz, offers sequence data on five strains of Ebola...outbreak and earlier ones, along with links to the relevant scientific literature.  -In light of the current...Medicine have made their complete collection of relevant papers freely available here. -PLoS and The New...

...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control...PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith Joung’... in human cells. Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper...

...typically grown in liquid culture without antibiotics to give the antibiotic resistance gene time to be expressed...being performed at Addgene, this adds up to a significant time savings. You can also save yourself time...homemade competent cells to ensure good efficiency.  Want even more tips for transforming your cells? Check...

...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...for a new genome-editing tool but it could be a fantastic add-on to a diagnostic toolkit, called DETECTR...

...a request and order if approved.  I want to stay updated!  We want to keep you updated! Subscribe to the...will be available for request in three different quantities: 10x 100 µL (1 mL), 25x 100 µL (2.5 mL), or 50x...process. This process will take 1–2 days, and we anticipate approving the vast majority of requests.  If ...

...ability to label live cells make this system truly advantageous. This new technique, developed in Thoru Pederson... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and... live cell imaging of genomic loci. Ma et al. anticipate that the technique will be applicable to studies...shattering (chromothripsis). As this study joins the pantheon of useful CRISPR/Cas9 techniques, we at Addgene...

...is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing...sequence. The spacer sequence is the RNA guide, like a wanted poster that Cas9 uses to track down its suspect—the... repeat section of the crRNA, forming the repeat:antirepeat duplex. tracrRNAs also contain multiple stem...have a blog post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got ...

...that had never been done at Addgene before. This meant I was responsible for establishing a new report ...certification on social media data, and I was given a fantastic spreadsheet template to build out a report on....more people who need it!  After a few months, we wanted to share these, and other, findings with the larger...to-dos, I also found time to go out to sushi restaurants for lunch with coworkers and visit the office...

... effective tools but neglect some biologically relevant situations. Tissue-specific genes – the genes...its functions can be observed. Functionally redundant genes – multiple genes which function in overlapping...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020. DOI: 10.1105/tpc.12.7.1007...

... be able to store your sample without having substantial degradation and which experiments you can use...preventing the activity of DNase. While this is very important to preserve the stability of your DNA, it will...elution of plasmid DNA through their kit. The advantage of using a Tris buffer is that it allows better...that elution buffer onto your DNA. Knowing how you want to use the plasmid DNA can help you choose the best...

...Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression...CRISPR is an incredibly fast-moving field, and we want to make it easy for you to keep up with new developments...editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3...

...or personnel to constantly prepare them for distribution. One of the major advantages to depositing materials...depositor’s dedication to scientific sharing, we wanted to celebrate our depositing PI’s who have the most...Given the increasing popularity of CRISPR in the plant community, this has saved us hours of work responding...