We narrowed to 897 results for: ANT;

... do not express Cre or Flp. Here at Addgene we quantify the amount of transgene recombination for every...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially...Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...

...component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended... Genome Portal at the University of California, Santa Cruz, offers sequence data on five strains of Ebola...outbreak and earlier ones, along with links to the relevant scientific literature.  -In light of the current...Medicine have made their complete collection of relevant papers freely available here. -PLoS and The New...

...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control...PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith Joung’... in human cells. Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper...

...typically grown in liquid culture without antibiotics to give the antibiotic resistance gene time to be expressed...being performed at Addgene, this adds up to a significant time savings. You can also save yourself time...homemade competent cells to ensure good efficiency.  Want even more tips for transforming your cells? Check...

...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...for a new genome-editing tool but it could be a fantastic add-on to a diagnostic toolkit, called DETECTR...

...a request and order if approved.  I want to stay updated!  We want to keep you updated! Subscribe to the...will be available for request in three different quantities: 10x 100 µL (1 mL), 25x 100 µL (2.5 mL), or 50x...process. This process will take 1–2 days, and we anticipate approving the vast majority of requests.  If ...

...ability to label live cells make this system truly advantageous. This new technique, developed in Thoru Pederson... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and... live cell imaging of genomic loci. Ma et al. anticipate that the technique will be applicable to studies...shattering (chromothripsis). As this study joins the pantheon of useful CRISPR/Cas9 techniques, we at Addgene...

...is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing...sequence. The spacer sequence is the RNA guide, like a wanted poster that Cas9 uses to track down its suspect—the... repeat section of the crRNA, forming the repeat:antirepeat duplex. tracrRNAs also contain multiple stem...have a blog post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got ...

... be able to store your sample without having substantial degradation and which experiments you can use...preventing the activity of DNase. While this is very important to preserve the stability of your DNA, it will...elution of plasmid DNA through their kit. The advantage of using a Tris buffer is that it allows better...that elution buffer onto your DNA. Knowing how you want to use the plasmid DNA can help you choose the best...

...that had never been done at Addgene before. This meant I was responsible for establishing a new report ...certification on social media data, and I was given a fantastic spreadsheet template to build out a report on....more people who need it!  After a few months, we wanted to share these, and other, findings with the larger...to-dos, I also found time to go out to sushi restaurants for lunch with coworkers and visit the office...

... effective tools but neglect some biologically relevant situations. Tissue-specific genes – the genes...its functions can be observed. Functionally redundant genes – multiple genes which function in overlapping...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020. DOI: 10.1105/tpc.12.7.1007...

...Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression...CRISPR is an incredibly fast-moving field, and we want to make it easy for you to keep up with new developments...editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3...

...or personnel to constantly prepare them for distribution. One of the major advantages to depositing materials...depositor’s dedication to scientific sharing, we wanted to celebrate our depositing PI’s who have the most...Given the increasing popularity of CRISPR in the plant community, this has saved us hours of work responding...

...Professor at the Lab for Innovation Science at Harvard, wanted to see how new tools drive innovation in synthetic...was moved exactly as is from one organism to a distantly related organism, the gene may not express well...showed a growing trend of sourcing genes from distantly related organisms. In 2006, less than 1% of the...it do? Those are the sets of questions one would want to have answered with a biosurveillance tool,” Kunjapur...

... drudgery to some scientists, MTAs are important—they guarantee certain rights and protections for depositor...another blog post: Why Do I Need an MTA Anyway?) Don't want to wait? Talk with your TTO Has a pending legal ...request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest that they get an Addgene...

... discipline and reduces the dissemination of unwarranted claims or unacceptable interpretations. A good...journal.  As a postdoctoral researcher studying plant development and abiotic stress tolerance, I have...peer-reviewer you wish for The last thing that I wanted to mention about the peer-review, is that as a ...ratio in Arabidopsis, (2) expression patterns in plants with enhanced sodium accumulation in their roots...

...updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic...one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene toxic...choice of ori depends on how many plasmid copies you want to maintain, which host or hosts you intend to use...amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number. Some ...

...minutes (incubating the full 30 minutes will not significantly improve the transformation efficiency).  5. ...primers; you need that free hydroxyl group! You may want to include extra extension time after the last cycle...mixing the PCR product with the TOPO vector, you may want to add extra salt to your reaction: Topoisomerase...next step before proceeding. Pre-warming your antibiotic-containing plate prior to plating your transformation...

...labs. We’re overjoyed to provide you with these fantastic educational resources and will be sure to keep...us at [email protected]. Thank you again for a fantastic year and happy reading! Top 10 blog posts of 2015...Addgenie Julian Taylor-Parker explains why you might want to use a specialized E. coli strain for protein ...Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired of having...

...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter ...apparent in metabolic engineering. The authors elegantly demonstrated the potential of this to control ...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...