We narrowed to 813 results for: Arc

...! First, a quick refresher: prime editing is a “search and replace” genome editing technique that relies...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

...sequences for Illumina sequencing as well as unique barcodes per sample so we can assign sequencing reads to...up the run making sure to note the appropriate barcode for each sample. About 24 hours later we unload...

...the number of genes that other classrooms and researchers can easily investigate in planarians. References...Alvarado, The American Biology Teacher, Vol. 79 No. 3, March 2017; (pp. 208-223). Link: http://abt.ucpress.edu...

...CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation with...decaffeination operon Article contributed by Angela Kaczmarczyk  Listen to the synthetic decaffeination operon...

...ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find ...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find...

...selection and expression in your target cell line, researchers often find themselves faced with a roadblock....cell line that is used for titering. Typically researchers will choose a relatively permissive cell line...

...refractory to gene delivery methods, but the researchers was able to edit hiPSCs, mouse bone marrow (BM...plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA delivery...

...excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity...fluorescence. Image from Eastman et al., 2020. The researchers tested several scenarios from embryo development...

...fluorescent proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs... brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered...

...mutant allele. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...traditional gene therapy pipelines (viral), and researchers are currently working on giving this treatment...

...targeting. By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific...complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino...

...gleaned from the literature and engagement with researchers who study the protein or structure of interest...introducing tags into your gene of interest. Researchers have developed a variety of endogenous gene tagging...

...This post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina ...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment...

...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone...

...Eric Green, Director of the National Human Genome Research Institute (NHGRI) of the NIH, started off with...acid science still has many surprises for the researchers in this field. Resources at Addgene Browse ...

...FRET based tools to study epigenetics Epigenetic research uses a wide range of molecular biologic techniques...none of these techniques can be used for in-situ research of dynamic modifications in real-time, posing ...

... the battle when using optogentics to answer a research question. The flip side of the optogenetics coin... molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007...

...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

...from one serotype and the capsid of another. Researchers have used these psuedotypes to further improve...-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S...

...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...