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We narrowed to 810 results for: Arc

Showing: 581 - 600 of 810 results
  1. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...mutant allele. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...traditional gene therapy pipelines (viral), and researchers are currently working on giving this treatment...
  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...targeting. By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific...complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino...
  3. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...gleaned from the literature and engagement with researchers who study the protein or structure of interest...introducing tags into your gene of interest. Researchers have developed a variety of endogenous gene tagging...
  4. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...This post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina ...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment...
  5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...sequences for Illumina sequencing as well as unique barcodes per sample so we can assign sequencing reads to...up the run making sure to note the appropriate barcode for each sample. About 24 hours later we unload...
  6. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity...fluorescence. Image from Eastman et al., 2020. The researchers tested several scenarios from embryo development...
  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...selection and expression in your target cell line, researchers often find themselves faced with a roadblock....cell line that is used for titering. Typically researchers will choose a relatively permissive cell line...
  8. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find ...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find...
  9. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...refractory to gene delivery methods, but the researchers was able to edit hiPSCs, mouse bone marrow (BM...plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA delivery...
  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone...
  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...Eric Green, Director of the National Human Genome Research Institute (NHGRI) of the NIH, started off with...acid science still has many surprises for the researchers in this field. Resources at Addgene Browse ...
  12. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...FRET based tools to study epigenetics Epigenetic research uses a wide range of molecular biologic techniques...none of these techniques can be used for in-situ research of dynamic modifications in real-time, posing ...
  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... the battle when using optogentics to answer a research question. The flip side of the optogenetics coin... molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007...
  14. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...
  15. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...from one serotype and the capsid of another. Researchers have used these psuedotypes to further improve...-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S...
  16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...
  17. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...
  18. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...University of California, Santa Cruz, where her research is focused on engineering nanoparticles as drug...
Showing: 581 - 600 of 810 results