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We narrowed to 856 results for: ELL

Showing: 581 - 600 of 856 results

  1. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...2016). It’s also been well established that recombinant antibodies are excellent for reproducibility because...protocols for recombinant antibody production as well as antibody applications. In addition, basic and... can transform the antibody sharing landscape as well.     References Andrews NP, Boeckman JX, Manning...

  2. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...graduate student studying plant development and cell-cell communication in the Nimchuk Lab in the Biology...untransformed seedlings or callus (undifferentiated plant cell culture), which is great for a wide variety of applications...observing RUBY compared to other markers lends itself well to nondestructive selection. If RUBY was integrated...

  3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In contrast...unable to show TtAgo-mediated cleavage in mammalian cells, likely due to high temperature requirements. To...plasmid and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9...

  4. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...planning for your visa and or green card process well before addressing your career alternatives in order...the United States including green cards that are well-tailored to meet the needs of researchers. The categories...related skills”. Many career paths in academia as well as the non-profit sector are transferrable to industry...national importance; (2) The foreign national must be well positioned to advance the proposed endeavor; (3)...below. An international or national award for excellence in your field. Membership in a professional organization...

  5. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...spend the afternoon lovingly tending to the stem cells I was using in an experiment, then spend the evening...evening listen to some bloke at the pub telling me that actually he drives better after a few drinks. A...it’s oddly fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen years...Kahan, D., Peters, E., Wittlin, M., Slovic, P., Ouellette, L. L., Braman, D., & Mandel, G. (2012). The polarizing...

  6. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...CRISPR to Cats Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton Learn How Plasmid...while some are looking to develop new skill sets. Kellen Andrilenas is a Ph.D. grad student at Boston University...engineering project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge...

  7. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... colleagues. When you attend a well-run meeting, note what worked well and ask the meeting leader for ...annoying interruptions to our “real” work. However, a well-run meeting can have quite the opposite effect. .... We’ve provided some tips above for what works well at Addgene, but meetings are never one-size-fits...

  8. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...expected to make sure the work gets done and done well. Involving your team effectively is now your new... in mind, you won’t be able to communicate these well to the task assignee and this is a recipe for bad...delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities, good ...Delegating" in the Harvard Business Review. Another excellent blog on delegation tips and practices can be found...

  9. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent... Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role..., Ensminger AW, Fox AH, Chess A, Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions...

  10. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... be wondering how this all fits in with science. Well, I’ll give you an example. At my previous company... goes into software product management? I always tell people the best part of my job is getting to talk...communicate with customers, software developers, as well as team members in automation, sales, marketing,...help you be a valuable asset to the scientists as well as the developers. Lastly, you’re going to need ...

  11. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...idea from academia to a startup Mike Koeris and fellow co-founders Tim Lu and others first came up with...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing...aligned in vision and outcomes. The team must work well together, have complementary skill sets, and “talk...were free and free to repair.” If the team works well, the idea is solid, and “the pitch is packaged to...

  12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...you correct any language- and spelling mistakes. A manuscript that is well written looks more professional...submission, missing files or a manuscript full of spelling errors will not have a great chance of getting...manuscripts submitted for publication. Holds a PhD in Cell Biology and a Masters degree in Infection and Immunity...

  13. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated by the ...to receive the Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also ... our most popular lentiviral and AAV vectors, as well as developing and sharing supportive educational...stories we can, and to hear the stories others have to tell.  "Also thanks for being Addgene. You guys rock....

  14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... channels in living cells. Multiple properties of a channelrhodopsin, such as cell localization, kinetics...would result in a functional channelrhodopsin, as well as predict the channelrhodopsin’s photocurrent strength...minimally-invasive optogenetics While ChRgers performed well in a tissue culture dish, that doesn’t guarantee...

  15. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...enable scientific reproducibility. This workshop was well received, and then when the team started brainstorming...widespread issue of irreproducibility. Use your network well to seek out valuable and impactful opportunities...course! Thanks to individuals in the group who were well connected, this diverse and international team assembled...website How-to-guide for Reproducibility workshops Levelling Up: Reproducibility for Everyone Addgene: a global...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...teams continue to keep up with the demand with excellence and dedication. We don’t have worry or wonder...right? Upgrades to the internal LIMS pages and excellent work on our external website appearance and functionality...support. The Addgene management team leads with excellence. They are one of the main reasons I have so few...Addgenies. I never worry about their dedication, excellent intentions, or incredible drive to succeed.  Thanks...

  17. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...read a 100-page genre fiction novella as it does to read an 11-page Cell paper - and sometimes, it might...obtain?” If the answer is yes, you will need to spell out that connection for your audience. I ask myself...needed. The trick is to communicate your key points well enough that the audience can take that knowledge...

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...generated against a linear antigen tend to work very well for applications that denature a protein to its ...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody...References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T,...

  19. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...practical solutions.  My PhD prepared me remarkably well for this task. Good scientists learn how to communicate...to say “I’m going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different...different types of “plates” (Fig. 1). When you have to tell an engineer that you want the LIMS software to create...It is never really done. But if you implement it well, here’s what can happen: More effective meetings...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...transfer 100 µl of glycerol stock to each of 2 96-well microplates (identical copies, see image above)....integrates our database of over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for... be found here. Finally, you will want to have a well-organized database of expected sequences, either...some tips on how to get better sequencing reads? Tell us your favorite sequencing tricks in the comments...
Showing: 581 - 600 of 856 results