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We narrowed to 889 results for: IND

Showing: 581 - 600 of 889 results

  1. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...scientists have yet to decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... my head and left me thinking: I wish I had this kind of experience earlier! After all, students build...PMID: 30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! ... Additional Resources on the Addgene Blog Find planarian tools for teaching developmental biology Use... Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here...

  3. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...DNA is harvested, much like one would prep an individual plasmid. However, lots of library DNA is needed...obtain about 100 to 1,000 bacterial colonies per individual plasmid in the library. Bacterial growth must...representing perfect uniformity (see Figure 3A). Addgene finds that this representation is most easily understood...amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped...

  4. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then ...recruits transcription machinery to the site to induce gene expression. Thus, genes and siRNA encoded ...neuronal compartment, you could express them individually or simultaneously. Do note for control purposes... much protein as overexpression of each gene individually. Applications of the Gal4/UAS system There are...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...motivated to do anything and everything we can to help find a cure -- and quickly. Normally drug discovery is...and help us figure out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6...over the world, as far flung as the EU, Russia, India and Saudi Arabia -- as well as closer to home in...QBI_UCSF and KroganLab), Instagram, and Facebook. Find more COVID-19 and coronavirus related plasmids and...

  6. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... monogenetic diseases, the bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR...single-guide RNA targeting the mutant allele (7). An independent team led by Hans Clevers at Hubrecht Institute...fibrosis report. “This is a big advantage over induced pluripotent stem cells, which need to go through...affects multiple organ systems. Nevertheless, the findings are proof-of-principle for use of CRISPR to correct...

  7. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...CD8 or CD4 epitopes, as well as their potential binding affinity to major histocompatibility complexes ...not yet possible for these algorithms to model individually-specific factors, such as previous exposures...prediction of immunogenicity is to validate the findings from the in silico assessments, as well as analyze...dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic in-vitro...

  8. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... has a web and desktop version that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero...desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero have a lot of ...two citation managers. If you want more tips on finding and organizing papers, check out this related blog... blog post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many...

  9. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ... Resources on the Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our ...paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable of forming...like an understatement. Multiple speakers from top minds in the stem cell field have described the Yamanaka...

  10. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ... and higher pH. We initially used this screen to find R body mutants that couldn’t extend at a normal ...the ciliate. It’s thought that they function as a kind of armor to protect their host from predation (Petroni....1021/acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...

  11. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...ssDNA molecules in vitro. Figure 1: Cas9 binding to the cleaved DNA duplex. Image from Richardson...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the...on Your HDR Basics Learn How to Design Your gRNA Find the Ideal CRISPR Software for Your Needs Resources... on Your CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our ...

  12. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... Podcast.       Resources at Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other...editing and screening) such that thousands of individual edits can be studied quickly. Involvement with.... the nonprofit, sharing ethic that Addgene has behind it is something that very much resonates with me...

  13. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...creates more breakthrough and innovation. With any kind of open platform, there is the potential for increased...general auditor. I oversee a department of 20 individuals that help the plan manage risk. My job and my...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...the other members of Addgene's Board of Directors Find careers at Addgene ...

  14. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...post is designed with beginners in mind, there’s some great reminders for Twitter pros as well, like tip...tips on using Twitter as a scientist. You can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage...capture your interest during that week.” You can also find Dr. Knoepfler on Twitter: @pknoepfle 3. "Social ...

  15. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...mutated cancer genes are rare and most of the individual variants found in tumors are exceedingly rare...But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit, the Broad...approach. Also, in principle, these experiments could find correlations between variants in a gene of poorly... next-generation of cancer patients. We hope you find these new cancer variant plasmid collections useful...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...(i.e. Csy4 cleavage sequence, tRNAs, multiple individual promoters).  spCas9 and its gRNAs are also larger...suitable substrate for Cpf1. This means Cpf1 can bind and cleave the lentiviral RNA, preventing packaging... with Cpf1 and GFP.  Of these neurons, ~15% had indels at all 3 targeted loci.  See the chart below for...Addgene.org Browse Our CRISPR Multiplexing Resources Find CRISPR Plasmids for Your Research Catch up on Your...

  17. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...You’ve committed to the daunting task of finding new factors in your biological system of interest, the...are assessed, regardless of whether they enhance, hinder, or have no effect on the process you are interested...everything else dies off. For a screen, you would individually treat cells with many different drugs and then... forward screens. A given panel of drugs are individually administered and then outcomes are measured....

  18. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...necessary for most ChIP experiences, but the increased binding can mask the epitopes antibodies recognize. It ...In the case of histone modifying proteins, which bind quite strongly to DNA, crosslinking may not be necessary...to the DNA, depending on how tightly the proteins bind natively. But the amount of cross-linking can affect...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...

  19. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...the Fc portion of different antibody isotypes. Binding to complement system proteins, which can initiate...chain domain as your primary antibody but doesn’t bind your antigen of interest. Isotype controls are used...staining you see is due to target-specific antibody binding rather than nonspecific background staining. Isotype...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant...

  20. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...processes (for example Dam and Dcm methylases) are independent from the restriction modification systems, yet...non-mutated and methylated template is destroyed leaving behind a pool of mutated plasmids which can later be verified...that is unmethylated at those sites. Please keep in mind that dam-/dcm- strains may have an increased rate...
Showing: 581 - 600 of 889 results