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We narrowed to 878 results for: IND

Showing: 581 - 600 of 878 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...DNA is harvested, much like one would prep an individual plasmid. However, lots of library DNA is needed...obtain about 100 to 1,000 bacterial colonies per individual plasmid in the library. Bacterial growth must...representing perfect uniformity (see Figure 3A). Addgene finds that this representation is most easily understood...amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped...

  2. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ... Resources on the Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our ...paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable of forming...like an understatement. Multiple speakers from top minds in the stem cell field have described the Yamanaka...

  3. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ... and higher pH. We initially used this screen to find R body mutants that couldn’t extend at a normal ...the ciliate. It’s thought that they function as a kind of armor to protect their host from predation (Petroni....1021/acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...

  4. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...ssDNA molecules in vitro. Figure 1: Cas9 binding to the cleaved DNA duplex. Image from Richardson...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the...on Your HDR Basics Learn How to Design Your gRNA Find the Ideal CRISPR Software for Your Needs Resources... on Your CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our ...

  5. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... Podcast.       Resources at Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other...editing and screening) such that thousands of individual edits can be studied quickly. Involvement with.... the nonprofit, sharing ethic that Addgene has behind it is something that very much resonates with me...

  6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...mutated cancer genes are rare and most of the individual variants found in tumors are exceedingly rare...But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit, the Broad...approach. Also, in principle, these experiments could find correlations between variants in a gene of poorly... next-generation of cancer patients. We hope you find these new cancer variant plasmid collections useful...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...(i.e. Csy4 cleavage sequence, tRNAs, multiple individual promoters).  spCas9 and its gRNAs are also larger...suitable substrate for Cpf1. This means Cpf1 can bind and cleave the lentiviral RNA, preventing packaging... with Cpf1 and GFP.  Of these neurons, ~15% had indels at all 3 targeted loci.  See the chart below for...Addgene.org Browse Our CRISPR Multiplexing Resources Find CRISPR Plasmids for Your Research Catch up on Your...

  8. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...creates more breakthrough and innovation. With any kind of open platform, there is the potential for increased...general auditor. I oversee a department of 20 individuals that help the plan manage risk. My job and my...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...the other members of Addgene's Board of Directors Find careers at Addgene ...

  9. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...post is designed with beginners in mind, there’s some great reminders for Twitter pros as well, like tip...tips on using Twitter as a scientist. You can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage...capture your interest during that week.” You can also find Dr. Knoepfler on Twitter: @pknoepfle 3. "Social ...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...necessary for most ChIP experiences, but the increased binding can mask the epitopes antibodies recognize. It ...In the case of histone modifying proteins, which bind quite strongly to DNA, crosslinking may not be necessary...to the DNA, depending on how tightly the proteins bind natively. But the amount of cross-linking can affect...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...

  11. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...the Fc portion of different antibody isotypes. Binding to complement system proteins, which can initiate...chain domain as your primary antibody but doesn’t bind your antigen of interest. Isotype controls are used...staining you see is due to target-specific antibody binding rather than nonspecific background staining. Isotype...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant...

  12. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...You’ve committed to the daunting task of finding new factors in your biological system of interest, the...are assessed, regardless of whether they enhance, hinder, or have no effect on the process you are interested...everything else dies off. For a screen, you would individually treat cells with many different drugs and then... forward screens. A given panel of drugs are individually administered and then outcomes are measured....

  13. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...processes (for example Dam and Dcm methylases) are independent from the restriction modification systems, yet...non-mutated and methylated template is destroyed leaving behind a pool of mutated plasmids which can later be verified...that is unmethylated at those sites. Please keep in mind that dam-/dcm- strains may have an increased rate...

  14. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...manuscript to make resources easier to identify, find back, and share. Depending on the type of manuscript...including F1000Research, also ask for the raw data behind the figures and results. This is a relatively new...researchers to access the data and make up their own minds on whether the data analysis has been of good quality...accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). ...

  15. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ... on the Addgene Blog Find tips for online social networking for scientists Find tools for using social...profile is absolutely necessary. If people can’t find you on LinkedIn, you will lose opportunities and...for politics, family updates, or click bait of any kind. For more on inappropriate content see this article...

  16. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...Addgene.org Find protocols for plasmid cloning Read our molecular biology reference Find how-to videos..., Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling...cloning strategy: Restriction enzyme digestion Be mindful of the restriction enzymes you chose. Make sure...

  17. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ..., the lack of caveolin 1 in breast cancer also induces changes of stromal cells in the microenvironment...transforming growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such...molecules that can be transferred to other cells, inducing epigenetic changes in targeted cells. In this ...our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...

  18. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...manuscripts shared by the authors on a public server independent from peer-reviewed publishing in a journal.  ...is usually a confidential process among a few individuals.  Early-career researchers especially enjoy the...an updated preprint, further increasing their findability. Now, go out there and finish that experiment...10.1371/journal.pbio.3000151 Sever R, Roeder T, Hindle S, Sussman L, Black K-J, Argentine J, Manos W, ...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... Five: Share your results.  Does this process remind you of anything? Perhaps the Scientific Method?...expected to write up a report to communicate your findings to your colleagues. So perhaps a better definition...time in a lab learning techniques and taking on independent projects, you will inevitably develop problem...improve. The next time you face a problem, try to be mindful of how you work through it. Ask yourself if you...

  20. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...numerical designations, separated by a slash, that indicate the ITR and serotype, respectively. For example...polarities are rescued from the vector plasmid and individually packaged into the AAV capsids. As a genome conversion...scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001)....Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All Viral Vector Resources ...
Showing: 581 - 600 of 878 results