We narrowed to 616 results for: LIS;

1. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

   Type

   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   ... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional...

2. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...

3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ...grant situation. So, young researchers need to publish good publications in a short period of time and...

4. Plasmids 101: Shuttle Vectors

   Type

   Blog Post

   Published

   May 20, 2025, 1:15 p.m.

   ...it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end there. Make sure...

5. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...

6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...

7. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ... a graphical representation of the results and a list of the analyzed samples (see below).   If the sample...

8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

9. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural...

10. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    Published

    Jan. 23, 2020, 2:45 p.m.

    ...these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest grains every time...

11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...Central PMCID: PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation...

12. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA...

13. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within cells. For example...

14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...

15. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...safe-targeting guides as a control will result in a smaller list of true positive hits than when using non-targeting...

16. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...

17. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...

18. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    Published

    June 28, 2016, 10:59 a.m.

    ... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such...

19. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...your own neuroscience sub-discipline that has published using the technique and ask if they would be willing...

20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    ... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,...