We narrowed to 677 results for: Mos
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TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m.... Developmental Biology meeting in Boston. The atmosphere was electric! After attending this meeting I ...
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Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....based on the cloning strategy you followed. While most of the troubleshooting regarding this step has to... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....stripped. HRP-RANbodies are, however, compatible with most multi-labeling immunohistochemical protocols where... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog PostPublishedFeb. 2, 2018, 3:20 p.m....write anything good again.’ ” For Susan, one of the most satisfying parts of the job is having completed ... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....proteins in situ immunocytochemistry is perhaps the most common approach. With a good and specific antibody... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....CRISPR-Cas9 to function on its target. They name their most potent Acr-LOV fusion protein inhibitor CASANOVA... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....consensus of academic stakeholders. However, while most institutions are indeed going ahead with their planned... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m.... AAV2/8 has a type 2 ITR and a type 8 serotype. Almost all vectors contain type 2 ITRs, which comes from... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m.... nanobodies, the project really took off. Unlike most antibodies, nanobodies, which occur naturally in... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m.... in the field of mouse transgenics. A few of the most common uses are listed below. Cre-dependent gene... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....response programs. NRs also encompass one of the most successful targets for drugs currently available... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....transcription start site will terminate transcription of most of the ncRNA (Figure 1D). While this method can ... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....store and share, making recombinant antibodies the most practical choice. Hybridomas are much tougher to... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....will not result in one complete, circular assembly. Most of the time, these plasmids are returned as one ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....Getting involved in science communication has been the most rewarding step I have taken in my career. The moment... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m.... with a gene of interest. These plasmids will be most useful for constructing intermolecular FRET probes... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....functional in many different human cell types, and while most of the genes we’ve tagged are widely recognized ... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....Interviewing remotely Remote interviews are likely new to most people - even hiring managers and your interviewers... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....for finding retinal cell-type specific promoters. Most existing promoters express in a broad number of ... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m....rAdV plasmid (~1 week): The AdEasyTM system is the most popular method for creating adenoviral vector constructs...