We narrowed to 801 results for: Ran
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TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....to choose Where is your audience? It’s, quite frankly, much easier to go where your audience is than ...resources do you have? No matter the project, I can guarantee you that either resource or time restrictions ...competitive while digital can be oversaturated. Transition from academic to informal writing can difficult...
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Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....jam packed with everything needed for synaptic transmission, are right around 250 nm, making it difficult...pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should...brief history and new avenues. Philosophical Transactions Royal Soc 380:20210110. https://doi.org/10.1098... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate... now connects MDH-focused CUREs at 50 schools, ranging from R1 institutions to community colleges, and...NADH production. CUREs are beginning to transform undergraduate education in the sciences by making... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....diligent scientist, they sought to understand these strange results by digging deeply into their data. ...Samples are divided into those of African ancestry(orange) and those of other ancestry groups (grey). ...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....enhancers and genome math come in. They help us to transform raw genomic coordinates into meaningful experiments...“GPS for proteins”) for NANOG, an essential transcription factor in embryonic stem cells (ESCs). Your ...as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong. In reality... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....control. A good positive control is bacteria transformed with the same backbone plasmid. If this control...strain. A good negative control strain is an untransformed culture of the same strain of bacteria you used...Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive Clones with Us ... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....responsibilities. Another tip: don’t miss your grandfather’s 100th birthday, as your mom will never forgive...when you can’t get home, babysitting or running errands. Give help when you have time, and in return you...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....result from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be ...file. On the plot, the green (edited sample) and orange (control sample) lines should be close together...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....paid on fellowships instead of grants such that PIs no longer save grant money by paying relatively low...the training they receive as postdocs is not transferrable to other environments. Slide from Paula Stephan... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....using the actual tool that you ordered and not a random aliquot pulled from a freezer. As you’ll see in...review our experimental designs and we need to be transparent about the reasons we alter these designs if we... wherein researchers can submit designs and transparently reveal them upon completing an experiment. There... -
CRISPR Guide Update
TypeBlog PostPublishedOct. 10, 2024, 1:15 p.m.... to use CRISPR plasmids in the lab and the vast range of possibilities at their disposal. With this update...CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....facilities management to website development, tech transfer, lab work, and more. It's this diverse group of...questionable collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words ... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog PostPublishedApril 1, 2021, 1:15 p.m....Subscribe to the blog to stay in the know! No pranks, just fun and games this April Fool’s Day on the... to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each photo, the pet... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog PostPublishedFeb. 6, 2018, 2 p.m....competitive field. Chapter 2: Wei Leong Chew's swift transition from graduate work to an independent position... Coming up with new project ideas, applying for grants, and mentoring. Chapter 5: Wei Leong Chew's lab... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog PostPublishedJan. 10, 2018, 4:13 p.m....Troubleshooting Viral Transductions Getting the Most from Your Lentiviral Transduction Additional Resources... -
Bringing Renewable Energy to Addgene
TypeBlog PostPublishedAug. 27, 2019, 1:08 p.m....100% renewable electricity! A (not so) shocking transition In early July the Town of Watertown announced...substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will allow us to... -
5 Science Rap Videos & Their Creator: Tom McFadden
TypeBlog PostPublishedFeb. 9, 2017, 3:30 p.m....the interview with Tom McFadden! 1. Rosalind Franklin vs. Watson & Crick - Science History Rap Battle...telling following the unfortunate story of Rosalind Franklin and her incredibly important role in solving the... -
CRISPR Cheat Sheet
TypeBlog PostPublishedJan. 27, 2021, 2 p.m....field moves fast. That’s why we’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook....other Cas proteins continue to expand the targeting range. CRISPR can be difficult to deliver. Not all cells... -
New Viral Vectors - Spring 2025
TypeBlog PostPublishedApril 29, 2025, 1:30 p.m....diverse needs of our community and support a wide range of experimental designs. Here are nine great additions...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure... -
2018 #DeckTheLab Contest Winners
TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....Sikora’s lab Matthew Sikora’s lab went all out to transform their lab into a walking tour of Buddy the Elf... care package from Addgene. We hope all of our entrants had fun decorating with their labmates! ...