We narrowed to 611 results for: SCR

1. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...

2. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...into E. coli harboring the defective CamR gene. Screening for surviving colonies and subsequent rounds of...

3. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...

4. Mesothelioma - Causes, Symptoms, and Treatment

   Type

   Blog Post

   Published

   Dec. 13, 2016, 3:30 p.m.

   ...Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional Resources on Addgene.org ...

5. The Challenges of Cell Culture

   Type

   Blog Post

   Published

   May 5, 2016, 2:30 p.m.

   ...free of contamination and match their original description. The oldest, largest, and most diverse of these...

6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ... live cells in real time. Previously, we have described a number of practical considerations that should...

7. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...

8. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   Published

   Nov. 5, 2014, 3:41 p.m.

   ...gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine...

9. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...the same). Examples of such targets include transcription factors, histones, and DNA repair enzymes. Note...

10. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    Published

    March 28, 2024, 1:15 p.m.

    ...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...

11. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ...probes are based on cofactor binding proteins as described above. The smallest tags are peptides that are...

12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...plasma membrane. In previous publications, they described tools to control formation of lamellipodia and...

13. Tips for Getting a Faculty Position

    Type

    Blog Post

    Published

    July 20, 2023, 1:15 p.m.

    ...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...

14. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...C-terminus of the fluorescent protein. I prefer to describe the different parts of the fusion from N- to C-terminus...

15. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    Published

    Sept. 29, 2020, 1:15 p.m.

    ...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...

16. Plasmids 101: Biotinylation

    Type

    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice...

17. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ...freeze all grants, contracts The Guardian: Trump to scrap Nasa climate research in crackdown on “politicized...

18. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    Published

    Aug. 23, 2016, 2:30 p.m.

    ...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...

19. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...PI on the panel even suggested projecting your manuscript onto a wall and reading it out loud. While it...

20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...