We narrowed to 600 results for: STO
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TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....) to name two more. Likewise, there are several store-bought cloning kits available which are all based...
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How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....laboratory research management consultant and coach in Boston, MA. He specializes in assisting research scientists... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....Symposium and #MeTooSTEM, academic bullies are hard to stop—they often have no boss or management structure ... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....re-cutting. Transposase removal of the cassette later restores the target sequence. This allows researchers to... -
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....to covalently link the backbone and insert and, PRESTO, you have a full plasmid ready to be used in your... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....distribution you are working with, graphing data with a histogram is sometimes sufficient to determine normality... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....move into science policy. Read broadly about the historical roots of science policy to connect to your why... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m....., Butler, R., Bewersdorf, J., Rothman, J. E., Johnston, D. S., Schepartz, A., & Toomre, D. (2019). Labeling... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....Targeted amplicon sequencing doesn’t tell the whole story Figure 1: Schematic representations of the... -
Antibodies 101: Flow Cytometry
TypeBlog PostPublishedJuly 20, 2021, 1:15 p.m.... then gate the B cell population and create a histogram of CD19-containing cells versus total cell number... -
Which Fluorescence Microscopy Techniques is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....Illumination (SIM), Single Molecule Localization (PALM/STORM), or Reversible Saturable Optical Linear Fluorescence... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....experiments. Immune response dynamics are much better understood thanks to these technologies but many questions... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....and for good reason. For the first time in the history of neuroscience research we have a technology that... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon... due to its condensed design. For both systems, custom spacers/gRNAs are easily inserted using BbsI and... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....from Nicole Gaudelli in David Liu’s lab in 2016. History of base transition editing Cytosine base editing... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....accurate Check plasmid sequence for frameshifts and stop codons Weird background patterns (dots, lines... -
22 Hot Plasmid Technologies from 2014
TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m....family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize...