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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 863 results for: TOR;

Showing: 581 - 600 of 863 results

  1. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...Engineering gut microbes in situ While the ability to monitor the microbiome with sequencing is vast, the ability...upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin with the...origin with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by... Counting cell division in the gut microbiota Monitoring microbial population dynamics in the gut can ...

  2. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...New Under The Sun Blog and is part of her larger tutorial series, A Beginner’s Guide to Writing Scientific...when I can’t it’s an easy search. Writing this tutorial has shown me that I’m still in the dark ages with...guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences at Louisiana...Manuscript by Maaike Pols, PhD, a Developmental Editor at F1000Research  ...

  3. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...compared to the information provided by the depositor. If a depositor provides a sequence for the plasmid, QC...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains... elements based on the information provided and vector type  Any tags, selectable markers, and fusion ...

  4. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...suite, with Beam Therapeutics initiating a base editor-based clinical trial for SCD in 2022 and Editas... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  5. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...student and I truly enjoyed learning.” Addgene’s Director of Product Management Eric Perkins, who has been...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal...Komuhendo, a Lab Technician, dreamed of becoming a doctor. After taking a year break in between undergraduate...interviewees. Fabienne Furt, Addgene’s Associate Director of Business Development, got her PhD in Plant ...

  6. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ a previous supervisor / someone...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...Networking for Scientists Series 4. Become a science communicator More and more fellowship applications are asking...on your grant application to your peers and collaborators can also help you get some critical insight ...

  7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ... infections along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential...plasmids from Addgene to construct NFIX expression vectors as well as reporters of NFIX function. They will...

  8. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...design was conceived by Mike Morrison, a psychology doctoral student at Michigan State University. The idea... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...be the focus of your poster - the data and your story. If you choose a single-color background but want...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ...depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi using four-dimensional contrast...assembly line for the creation of CRISPR targeting vectors that each contains a guide RNA and repair template...  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc- 119 +...

  10. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...subcloned (using FX cloning) into the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag... and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound spike... Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101....16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L...

  11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...daytime. They mostly stop emerging at night. An incubator with a light cycle makes for very predictable ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that...research moving. Below, you’ll find some great introductory resources. As you dive deeper into Drosophila... the upper right hand corner. They have video tutorials under the help tab like this one to get you started...

  12. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...to) feel valued. Talk to your manager and find a mentor Your manager should support you, just like you ...reaching out to your manager. This is when having a mentor - someone more experienced to guide you - is valuable...Management for Scientists Series What Makes a Good Mentor? Tips for Working in Industry and Success in Management...

  13. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...thankfully, most competent cells do not express this factor. Zeocin is handy because it can be a one-stop shop...Hadley, W. K., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial...bonded proteins in its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-...

  14. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

  15. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...team compares it to the plasmid information or depositor-provided sequences to confirm the correct plasmid...them. DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the... full-length predicted sequences submitted by depositors. A sample will automatically pass through our...(1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences...

  16. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...bacteria, however, the protein integration host factor (IHF) assists with the process: it bends the DNA...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...nar/gky457 Park, K., Han, E., Paulsson, J., & Chattoraj, D. K. (2001). Origin pairing (‘handcuffing’) ...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... weird black pellet's DNA yielded a new CRISPR effector protein they named Cas7-11. Testing showed that.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...single protein, shedding the complexity of its ancestors. They classified the system as Class I, Type III-E...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature. 2021 Sep;597(7878):720-725. doi...

  18. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...possess resistance genes survive and multiply. Factors contributing to the development of resistance include...www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely take time to determine if ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

  19. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...due to loading and transfer errors — not to any factors from before the proteins even hit the gel. But ...all, while overloaded proteins may saturate the detector. In between these extremes lies the linear detection...concentration as well as by antibody dilution — both factors we’ve covered in our previous blog posts (1, 2)...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...have previously used histone deacetylase (HDAC) inhibitors to increase transcription of the integrated HIV...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...TALENs focused primarily on disrupting CCR5, the receptor that HIV-1 uses to enter a cell. ZFNs and CRISPR...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...
Showing: 581 - 600 of 863 results