We narrowed to 642 results for: Vars;

1. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...MOI optimization to account for batch-to-batch variability. Check out this post to learn more about different...

2. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ...versatile and efficient way to generate a wide variety of constructs. Similar, Gateway cloning (discussed...

3. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ... this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate...

4. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...for easy screening of the insertion event, and a variety of connector modules (linker sequences between ...

5. PhD Applications After COVID

   Type

   Blog Post

   Published

   Jan. 5, 2023, 2:15 p.m.

   ...are unable to attend in-person interviews for a variety of reasons (logistical, financial, etc…) now have...

6. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...nuclease-induced gene disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al...

7. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   .... Despite the prevalence of Ras mutations in a variety of cancers, Ras proteins have stumped the pharmaceutical...

8. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...

9. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP....

10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ..., and the balance of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a...

11. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    Published

    Feb. 4, 2016, 3:30 p.m.

    ...Check the Price of course! The price of some items vary, for example, plasmid kits are avaliable for less...

12. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...modifying the genome. If you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR...

13. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    Published

    July 16, 2015, 2:30 p.m.

    ... reviews on several other LSFM configurations’ various features see (4,7).   Light sheet fluorescence ...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...BiFC experiments. The ideal control is a mutated variant of one or both of your PIPs in which the protein-interaction...

15. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    Published

    Jan. 16, 2020, 2:18 p.m.

    ...ddPCR. One issue with qPCR is that the results can vary by a factor of 2. This means that if you set up ...

16. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...different genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...

17. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ...researchers have begun to make important discoveries in various biological fields. In 2015, the Fujii Lab identified...

18. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ... or affinity tags (HA, FLAG). There are also a variety of plant selection markers (basta, hygromycin, ...

19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with...

20. CRISPR Antimicrobials

    Type

    Blog Post

    Published

    May 3, 2016, 2:30 p.m.

    ... based on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their ...