We narrowed to 906 results for: addgene
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TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted.../s41587-020-0453-z Additional resources on the Addgene blog Browse our blog posts on base editing Read... in our CRISPR 101 blog series Resources on Addgene.org Find base editors for your research Read our...
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Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....additional pooled CRISPR libraries, also available at Addgene, enabling systematic investigation of the cellular...the use of pooled libraries in your experiments, Addgene recommends that you review the steps for how to... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog PostPublishedSept. 24, 2020, 1:15 p.m....2020 This post was contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist ...find validated gRNAs for your next experiment in Addgene's Validated gRNA Sequence Datatable. A more extended...://doi.org/10.1093/nar/gkt555 Resources at the Addgene Blog Listen to our Podcast with John Doench Learn... the Broad Institute and has worked with many Addgenies to help improve the understanding, curation, and...Screens Read other CRISPR Blog Posts Resources on Addgene.org Brush up on Your CRISPR Basics with Our CRISPR... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....Resources on Addgene.org: Check out the Addgene AAV guide Find ready-to-use AAV preps from Addgene's viral ...10.1093/hmg/ddv386 Additional resources on the Addgene blog: Find tips for a first time AAV user Learn... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....TALEs), with fifteen total variants deposited with Addgene. These variants, including a “miniCRISPR” version...popular target for labeling these cells. Check out Addgene’s Neuroscience Antibody Collection to find a recombinant...patterns from recombinant antibodies produced at Addgene (N206A/8R) were compared to the hybridoma-derived... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac... to help us expand this useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase...PMCID: PMC3627465. Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase...Repair Is Used for Genome Editing Resources on Addgene.org Find Vectors with the piggyBac™ Transposon Catch... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon... in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use...CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage...CRISPR system in E. coli. The system available from Addgene consists of two plasmids: pCas9: carries Cas9 ...://doi.org/10.1093/nar/gkw625 Resources on the Addgene Blog Check out our CRISPR Featured Topic Page ... out about CRISPR Multiplexing Resources on Addgene.org Browse our CRISPR Guide Pages Find Bacterial... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m....appreciate the humor in science and in the lab? Addgene is after all a place that encourages people to ... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....PubMed PMID: 20036813. Additional resources on the Addgene blog Read our cancer related blog posts Learn ... Find plasmids for your research Learn about Addgene's viral service ...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m.... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858. Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....optogenetics Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors.../s41592-019-0583-8 Additional resources on the Addgene blog Read more about the PHP.eB AAV capsid Read... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....s41586-021-03886-5 Additional resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for ...beyond spCas9 Cas proteins More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....few of the newest CRISPR plasmids available at Addgene. If you want to read more content related to CRISPR...PEDAR plasmids Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by... Various Addgenies... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...s00018-019-03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m.... 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide AAV Viral Preps References Chen...Resources and references More resources on the Addgene blog Viral Vectors 101: Viral Vector Elements Viral... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....) Golden Gate assembly (backbones available at Addgene) with examples of potential synthetic chromatin...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... Various Addgenies... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....PMCID: PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed Boyden Bernardo Sabatini Wenbiao...Wickersham Hongkui Zeng Additional Resrouces on the Addgene Blog Read about Other Means of Genome Engineering... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m....are publicly available, including those found at Addgene! Figure 1: Strategy for assembling the barcoded...PubMed Central PMCID: PMC4780610. Resources on the Addgene Blog Learn Best Practices for Designing Your gRNA...about CRISPR/Optogentics Tools Resources on Addgene.org Catch up on Your CRISPR Background with Our ... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide... PMCID: PMC514534. Additional Resources on the Addgene Blog Read Our Lentivirus FAQ Learn How Lentivirus... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...