Skip to main content
Addgene
Showing: 581 - 600 of 793 results

  1. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... wells with a purified protein allows you to characterize properties of an antibody that recognizes that.... As mentioned above, it can also be used to characterize antibodies against the antigen bound to the ...

  2. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...With the pseudovirus, scientists can study the mechanisms of infection and how to interfere with it in ....org/10.3390/v12050513 Hsieh C-L, Goldsmith JA, Schaub JM, DiVenere AM, Kuo H-C, Javanmardi K, Le KC, ...

  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...sequencing of neurons from each class to fully characterize the transcriptome. The first data release for...C.elegans neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle ...

  4. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...derived from random crosses between mice of uncharacterized backgrounds to create a limited but genetically...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to ...

  6. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...semi-circle (so they can see each other) on comfortable chairs (stools if you have to, but this is tough for women...things started. Give a couple of audience members a chance to participate. For example, at a recent event ...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants...

  8. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...cells for long periods of time. This reduces the chance of integration at random locations that could cause..., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J....

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...time consuming to produce, so most labs opt to purchase adenoviruses from dedicated adenoviral production...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Martin, B. K., Calderon, D., Lee, C., Choi, J., Chardon, F. M., McDiarmid, T. A., Daza, R. M., Kim, H.,...Vergara, X., van der Weide, R. H., Morris, B., van Schaik, T., Manzo, S. G., Peric-Hupkes, D., van den Berg...

  11. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since the discovery... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

  13. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...Knockout plasmids Homologous recombination is a mechanism to accurately repair harmful double stranded breaks... breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of DNA...

  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...setting and no manager will ever be perfect, but the chances of getting a good manager do tend to be higher ...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...

  15. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... tRNA synthetases (aaRS) have been created, characterized, and validated by the Gräslund lab. Recent findings...candidates using a synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...individual cell clones with different expression characteristics from a population of transfected clones. The...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...attending on the larger side? At large conferences, chances are that several talks will be going on at once... for myself like meet ten new people each day. Exchange business cards. When you’re done networking for...

  19. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...are they different from mine? What equipment purchases do we prioritize in a lab with both electrophysiologists...Collide: Managing Successfully Across Cultures by Richard D. Lewis Additional resources on the Addgene...

  20. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...heard of another double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ...of a single microhomology sequence. For more mechanistic details on HDR and NHEJ, please see the linked...
Showing: 581 - 600 of 793 results