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Showing: 581 - 600 of 766 results

  1. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions about...social media and blog posts, lesson plans, or information briefs for policymakers. The rapidly changing...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed... for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...published first. Because behavioral optogenetics is performed in freely moving animals, the fiber optic patch...into some of the important considerations when performing optogenetics behavioral experiments. Thank you...

  4. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

  5. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...over mVenus and 4-fold over the previous best performer mGold — while maintaining high brightness and ...Emily P. Bentley While several viral tools can perform neuronal tracing, anterograde tracing poses challenges...

  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...

  7. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to...Allen Institute’s recent blog post. For more information on other CRISPR/Cas systems used for endogenous...

  8. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from dividing ...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...of droplets) can be sufficient to capture the information needed for your PCR experiment. The process to...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...the standards versus the response and use that information to extrapolate the concentration of an unknown...generated by replicate values provides useful information about the accuracy of the curve, which we will...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...system as Class I, Type III-E, and then used this information to identify a large variety of related bacteria...

  13. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... these steps, we discover we need additional information. It is quite possible that the clarifications...hows and whys of our users, we are able to make informed software design choices, resulting in software...

  14. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...documents into RSpace, maintaining the original formatting. In this way data is moved from a static and ...conversations and import them into RSpace. The kind of informal conversations about research that can take place...

  16. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...resources and databases are scrutinized, and this information is enriched with your technical knowledge and...data sets, troubleshooting protocols, analyzing information, and solving customer problems. Technical support...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...called CROPseq-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

  18. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...

  19. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...passage the cells (as well as recording this information in your lab book and on the actual flasks of ...

  20. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139... T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have p15A origin of ...
Showing: 581 - 600 of 766 results