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...vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the...

...whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...

... all three edits and were thus more efficient. Informed by these mutations and previously described TadA...98.3%, or 98.6% efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold. The ability of...

...details below) can also be adapted to a competitive format. Figure 2: Overview of using Competitive...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

...as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed... for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM...

...published first. Because behavioral optogenetics is performed in freely moving animals, the fiber optic patch...into some of the important considerations when performing optogenetics behavioral experiments. Thank you...

...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to...Allen Institute’s recent blog post. For more information on other CRISPR/Cas systems used for endogenous...

...over mVenus and 4-fold over the previous best performer mGold — while maintaining high brightness and ...Emily P. Bentley While several viral tools can perform neuronal tracing, anterograde tracing poses challenges...

... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...

...growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from dividing ...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...

...list will let you jump directly to the type of information you are looking for in our catalog or educational...a simple search. These pages provide helpful information about the subject, link to related guides and...

...the standards versus the response and use that information to extrapolate the concentration of an unknown...generated by replicate values provides useful information about the accuracy of the curve, which we will...

...of droplets) can be sufficient to capture the information needed for your PCR experiment. The process to...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...

...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...system as Class I, Type III-E, and then used this information to identify a large variety of related bacteria...

...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...

...resources and databases are scrutinized, and this information is enriched with your technical knowledge and...data sets, troubleshooting protocols, analyzing information, and solving customer problems. Technical support...

...called CROPseq-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...et al., Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010...

... these steps, we discover we need additional information. It is quite possible that the clarifications...hows and whys of our users, we are able to make informed software design choices, resulting in software...