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Showing: 581 - 600 of 772 results
  1. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions about...social media and blog posts, lesson plans, or information briefs for policymakers. The rapidly changing...
  2. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...time of synthesis and, after a few hours, a conformational change causes the protein to fluoresce red....lentiviral preps in addition to the pooled library format! The library inhibits over 18,000 human genes. ...
  3. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes) A Near-Infrared BiFC ...Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes) Bright monomeric near-infrared...
  4. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...details below) can also be adapted to a competitive format. Figure 2: Overview of using Competitive...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...
  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the...
  6. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...
  7. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed... for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM...
  8. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...published first. Because behavioral optogenetics is performed in freely moving animals, the fiber optic patch...into some of the important considerations when performing optogenetics behavioral experiments. Thank you...
  9. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to...Allen Institute’s recent blog post. For more information on other CRISPR/Cas systems used for endogenous...
  10. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...
  11. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...
  12. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...over mVenus and 4-fold over the previous best performer mGold — while maintaining high brightness and ...Emily P. Bentley While several viral tools can perform neuronal tracing, anterograde tracing poses challenges...
  13. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from dividing ...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...
  14. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...of droplets) can be sufficient to capture the information needed for your PCR experiment. The process to...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...
  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...the standards versus the response and use that information to extrapolate the concentration of an unknown...generated by replicate values provides useful information about the accuracy of the curve, which we will...
  16. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...resources and databases are scrutinized, and this information is enriched with your technical knowledge and...data sets, troubleshooting protocols, analyzing information, and solving customer problems. Technical support...
  17. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...
  18. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... these steps, we discover we need additional information. It is quite possible that the clarifications...hows and whys of our users, we are able to make informed software design choices, resulting in software...
Showing: 581 - 600 of 772 results