Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 833 results for: ins

Showing: 581 - 600 of 833 results

  1. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...favoring the separation of the two helixes. Low pH instead results in the breakage of the ester bonds responsible...the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification are endA negative...mutated to neutralize the nuclease activity. For strains that still carry this gene, there are usually extra...

  2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...is expressed. In cells where the stop cassette remains, the effector is not expressed and the cells are...leads to gene constructs where the stop cassette remains following recombination. This strategy results ... Aliyah Weinstein...

  3. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...bacteria. Until recently the only available Cas9 proteins were isolated from mesophilic (medium heat loving... as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at high temperatures, so to use...when incubated in diluted human plasma. GeoCas9 remains active after being incubated in up to 30% human...

  5. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...use two antibodies - one against your target of interest and a second against the first antibody. When.... (C) A third solution is to use Fab fragments against the sample species to block the binding sites on...

  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...this is a much-needed community resource. Instead of painstakingly searching for experimental details in ...serotype, promoter, or tool type and each entry contains an image along with key experimental details (...

  7. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this person ...the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled. ...interviewed for 1 hour so having a broad enough insight was going to help me push both my scientific and...

  8. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...platform to help researchers working on COVID-19. Its origins trace back to March 2020, when the first coronavirus...use this potential to make a difference not just against COVID-19, but also in other areas of scientific...Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL...

  9. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ... of cell culture medium, which is buffered and contains the pH indicator phenol red. We froze samples ...der Geer P, Manning MC, Fitchmun MI. Protein instability following transport or storage on dry ice. Nat...10(4):278-9. PubMed PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman...

  10. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...was robust, capable of generating a wide range of insert sizes and also produced high yields of quality ... an NEB drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the...being overwhelmed by all the new information, and instead allow me to pay attention and answer my key questions...

  11. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ... became our bacterial transformation guru and instructor for our intro to biohacking courses. Our members...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

  12. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...DSB) Cas9 and its variants have two endonuclease domains: the n-terminal RuvC-like nuclease domain and the...conformational change that positions the nuclease domains to cleave opposite strands of the target DNA. Thus...refer to this RNA complex as the “gRNA”. The gRNA contains both the 20 nucleotide target sequence to direct...

  13. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...system less bulky and easier to use with other strains of E. coli, Schmidl et al. condensed the system...ensures that they can be used with many types of proteins, including Cas9 and dCas9-transcription factors... optimal. These tools are compatible with many strains of E. coli, as well as with other plasmid-based...

  14. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...available from your institution, your department, and online. The Howard Hughes Medical Institute (HHMI) offers... to an end and your next professional adventure begins. While many career pathways exist for scientists...

  15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA is an online, searchable...structure junky who just wants to learn about Cas domains. CasPEDIA either directly has this information ... More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s digital resources in...

  16. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...impractical for small projects. Most researchers instead turn to so-called “mismatch nuclease” assays (e.g...then performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time...your genome edits, this paper provides detailed instructions on how to design such assays, in addition to...

  17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ... ABEs are only weakly compatible with many Cas domains beyond SpCas9. In order to expand the gene-editing...The latest ABE from the Liu lab is ABE7.10 which contains the deoxyadenosine deaminase, TadA-7.10 (Gaudelli... of naturally occurring enzymes and functional domains speed up the development towards faster and more...

  18. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...well as related items (for example, a kit that contains a plasmid with the catalog ID). Depositor suggestions...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ...

  19. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...interaction between neuroscience and other fields. For instance I met one researcher studying how the microbiome...’ve developed in your current work to make new insights in neuroscience. Even if you're currently a synthetic...select viral vectors in the repository, you can instead have Addgene make the virus for you. Neuroscientists...

  20. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...succeed in communicating. Even the most rigorous, insightful, and novel scientific research will be wasted...research proposal is the perfect fit to their institutional focus and that you're better equipped than anyone...with ComSciCon, find us on Twitter, Facebook, Instragram, or send an email to [email protected]. Many...
Showing: 581 - 600 of 833 results