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  2. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...typical research project can take several years to complete and has multiple benchmarks. Think about what ...Researchers should  pick a topic that “looks at the complexity of biology and break apart a piece that can address...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where You Can Find...

  4. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...cannot be indirectly probed simultaneously on the sample. What to do?  If you’ve ever found yourself facing...visualize both targets in parallel (Fig. 2). For example, if both of your antibodies are rat IgG1, then ...

  5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...(so called “gDNAs”) rather than the RNA guides employed by Cas9 or Cpf1. These short, 24 base DNA guides...

  6. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ..., this cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing...found in the metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments...

  7. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...efforts is the Golden Stopwatch Award to celebrate exemplary TTOs. Our new Golden Stopwatch Award recognizes...agreements slow down your research; provide specific examples if possible Tell them about the Golden Stopwatch...

  8. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Pharmaceutical in 2017. As one of the original employees at Dimension Therapeutics, she helped build and...manufacturing and quality, especially with regard to compliance with health authority regulations,” Baradaran...

  10. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...educational materials to our repository of open samples. We are, and have always been, a community resource....43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility through Rigor...

  11. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ..., in turn, the big picture of the project. For example, how can future regulations impact your work? What...sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular activities The table below...

  12. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...from a month to a year. The researcher remains employed by their home institute, meaning the host university...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...

  19. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ... binding affinity to major histocompatibility complexes (MHC class I and II).  There are multiple algorithms...approach The in silico approach cannot replicate the complexity of antigen processing events that take place ...

  20. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer...
Showing: 581 - 600 of 778 results