...build a strategic network to facilitate your company’s development  Don’t be afraid to email strangers asking...likely need a functional prototype to gain any real traction.  This can carry a heavy price tag, especially... to the healthcare landscape.  You will find an extra layer of difficulty launching a company in the health...for internal operations and building external strategic partnerships with universities, companies, hospital...

...just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew it was...candidate, I knew they would look for a candidate with extra experience or a marketing interest that would be...scientists. Tailoring my CV for the job To become an attractive candidate I knew I had to push not only my academic...I had no previous business, marketing or sales training and knew I had to start somewhere. I knew if I...promotions or further education may allow you to transition into various realms of business.  Thank you ...

...ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well as mammalian and human cell...conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently...the Tara Oceans Project datasets. This study demonstrates the power of combining the analysis of publicly...are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time...

...setting writing goals, tracking progress, and starting a writing group. Loop Habit Tracker app for Android  ...to get those words out and onto the screen and transform your work into a paper or a grant. Here’s a list...  This app lets you track habits, like writing. It’s basically the equivalent of crossing off days on ...I like to look at these graphs when I need that extra push to stick with my writing routine. The graph...

...biology labs the world over. Scientists have traditionally used Sanger sequencing to validate these constructs...to reconstruct the full individual sequence. Traditional NGS workflows are limited by costly library prep...normalized across a wide range of input DNA concentrations. This process is explained more fully in the...numbers of samples with a wide range of input concentrations (via proprietary chemistry). This turns out...shown in Fig. 2, below, differences in input concentration across a 100-fold range (1ng – 100ng) are normalized...

...Choosing a lab can be a major decision. A science trainee will spend 4-7 years working for one person and...you’re within a year from the end of your PhD or contract, you should be starting to send out polite emails...important, the end goal during this time is to be trained to think and execute tasks like a scientist. Of...where the topic of your research can make your transition easier – choose a lab that works on problems ...can. You know you are in the zone when you lose track of time because you are engrossed in a task. Things...

...Wen et al., 2018). SRIRACCHA uses the Piggybac transposon to stably integrate a reporter gene that contains..., you’re ready to edit. These cells are then transfected with several different constructs for the reporter... gRNAs by simply changing the gRNA used when transfecting your cells.  A plasmid that contains Cas9. The...plasmid.  With this set up, all successfully transfected cells will express RFP while cells containing... Lastly, once a mutant is identified, the PB transposase can easily and seamlessly remove the integrated...

...also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing ...native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of an error-prone... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...19.1 (2018): 143. PubMed PMID: 30253789. PubMed Central PMCID: PMC6154929. Halperin, Shakked O., et al....

...selection is a process in which only cells with the trait of interest will survive in the selected growth ...unknown manipulation of the population. This is contrary to reverse screens, where there is removal of ...usually selected. The method you choose to assess traits of interest is highly specific to your scientific...the end of the selection process. Non-functional traits will be automatically eliminated, unlike in screens...Directed evolution of enzymes: Library screening strategies. IUBMB Life, 61: 222-228 (2009). doi: https:/...

...556.7699 (2018): 57. PubMed PMID: 29512652. PubMed Central PMCID: PMC5951633. Lee, Jungjoon K., et al. "Directed....1 (2018): 3048. PubMed PMID: 30082838. PubMed Central PMCID: PMC6078992. Nishimasu, Hiroshi, et al. "...2018): 1259-1262. PubMed PMID: 30166441. PubMed Central PMCID: PMC6368452. Strecker, Jonathan, et al. "...10.1 (2019): 212. PubMed PMID: 30670702. PubMed Central PMCID: PMC6342934. Teng, Fei, et al. "Repurposing... 4.1 (2018): 63. PubMed PMID: 30510770. PubMed Central PMCID: PMC6255809.  Additional resources on the...

...brightness When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent...experimental conditions that are related to the imaging strategy and the sample. Practical brightness A protein...developed in our lab uses a reference protein that is translated from the same open-reading frame and separated...ranking of practical brightness can be made. This strategy has been used in yeast by Lee et al (2013) and...

...scientists and the support of their technology transfer offices, Addgene would not be able to have such...to recognize a few scientists who have gone the extra mile for this particular project. John Doench and... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...inventory management systems to enable efficient, tracked production and distribution of viral samples. The..., a big thank you to the Addgenies who went the extra mile to create this new service. We knew the scientists...

...modeling work in human pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit... designer proteins that will introduce a double-strand break in the genome anywhere you would like. Cells...cells after the plasmids have been introduced by transfection or electroporation. All you do to create a protein...not even five years ago, scientists relied on traditional homologous recombination. That technology had...the target site. Also, any time you create double-strand breaks, there is a possibility of introducing a...

...interaction (PPI) studies in plants that rely on transient transfection systems using Agrobacterium (such as Nicotiana...plasmid (Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning, the GOI first needs to be cloned...al., 2016)). During Agrobacterium-mediated DNA transfer, each GOI is usually expressed from a different...plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

... realized that running interactive workshops to train researchers on tools and best practices could be...were covered in the workshop. These workshops illustrate the open and modular nature of the workshop content... post-workshop surveys, recording results, and tracking material downloads. This was particularly helpful...it was realized that interactive workshops that train and provide researchers with tools and best practices...on the Addgene blog: Check out this guide on Transferable Skills: External Collaborations Learn about ...

... the effort associated with a specific topic; extraneous cognitive load refers to the way information ...every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...summarize the concepts you’re covering. Don't show frustration if they're incorrect; take it instead as valuable...similar misconceptions, it’s time to rework your strategy. Don’t assume it’s the audience’s fault; instead... the information. Maybe I needed to remove a distraction, such as pausing or turning off the slide deck...

...mutations, we check to see if they affect the translated amino acid. We also confirm that the species ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Pooled libraries are a bit more challenging to... work with the depositing lab to develop a PCR strategy suitable for the library in question. We will ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org...2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc 12:828–863...

...including excision or insertion, inversion, translocation or cassette exchange. By utilizing recombinases...recombinase activity. Opto-Cre-Vvd is precise, demonstrating low sensitivity to ambient light, and versatile...-TBN-3’ (where B is G, T, or C). The authors demonstrate that CRISPR-Casɸ is active in vitro as well as... more compact genome editors due to delivery constraints and scientists seeking a wider range of targetable...-based Dual-fluorescent DSB Repair) is a double-strand break repair assay for mammalian cells. It's based...

...genetics in the media (both traditional and social); and synthesizing and translating all these into social ...number of different opportunities to broaden my training beyond the biological sciences, as well as to ...communication experience. These included taking extra coursework and attending seminars in bioethics, ...Find all of our science communication posts Extracurricular activities for a strong science career path...

...fiber optic for the implant depends on the dorsal/ventral location of your region of stimulation (the z-axis...photons to become activated. Light is able to penetrate brain tissue a short distance so ideal implant...jbc.M111.253674. PubMed PMID: 21628464; PubMed Central PMCID: PMC3138296. 2. Sparta DR, et al. Construction...0471142301.ns0216s58. PubMed PMID: 23042500; PubMed Central PMCID: PMC3466483. 4. Kim TI, et al. Injectable...science.1232437. PubMed PMID: 23580530; PubMed Central PMCID: PMC3769938. 5. Iwai Y, et al. A simple head-mountable...