...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection...enough to get a sufficient titer; this is often the case with large genes. This is due to inefficient packaging...inserts; a study by Kumar et al. found that in some cases, titers can drop 10-fold for every 2kb increase ...

...other things, science communication comprises broadcasting, science writing, and certain aspects of art...audio or visual person. Why don’t you record a podcast or put some effort into making a video? Use your...Communication Posts Browse our Career Page Listen to Our Podcast Episode on Science Communication Additional Resources...

...they’re knowingly selling a bad product (in which case, you should probably stop using that company). Frequently...frequently asked by customers (and I expect this is the case at most companies). Always go back to the source...that could take weeks to resolve adequately. Occasionally there are some really off-the-wall questions...

...those used for breeding heterozygous mice. In this case, your parental mouse should be hemizygous at the...similar epigenetic modifications. Special breeding cases Whether your mouse colony consists of genetically...explained more in Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want ...

...your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit.... "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics...blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog posts...

...endoplasmic reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro...developed almost 40 years ago? Well…kinda. In some cases, antibody providers are distributing monoclonal ...same hybridoma developed by Evans, et al. In other cases, providers have moved to producing anti-Myc [9E10...

...always maintain a backup replicates of each clone in case of contamination. We do not normally use antibiotics...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...potentially large number of single clones obtained (on occasion we have prepared two 48-well plates of clones)...

... matches the copy number of your sample. In that case, an RQ value of one (or, more realistically, between...ddPCR may be a better option for more challenging cases.  Price point If budget is a concern, look for...number and it may not be able to identify trends in cases where the copy number variation is two or less (...

...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

...compared the number of plasmid orders and new COVID-19 cases for each week by location. We observed that as expected...drop dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining...map showing the plasmid orders and new COVID-19 cases each week. Statistical Modeling Note -- If statistical...