We narrowed to 803 results for: Cre

...visibility is increasing internationally, thanks to evolving outreach tactics, increasing business development...they see the value in depositing and they are increasingly seeking us out on their own to share reagents... We were and remain ahead of most companies in creating a welcoming, inclusive workplace for everyone....about their dedication, excellent intentions, or incredible drive to succeed.  Thanks to all the Addgenies...

...Foundation, and Stanford University—that harnesses the creative power of sex and gender analysis for innovation... in research Governments, universities, and, increasingly, corporations in the US and Western Europe have...decades: “Fix the Numbers of Women” focuses on increasing the numbers of women participating in science...gender analysis and 2) provides case studies with concrete examples of how gender analysis leads to discovery... Environment—a project aimed at harnessing the creative power of sex and gender analysis for innovation...

...these demands? Are there other solutions, such as increasing numbers in Master’s level programs? What would...Maryland College Park, is a strong supporter of increasing the number of postdoctoral scientists at University...School. He has continually promoted the cause of increasing data collection about the scientific workforce... Boston 2015 where we hope to begin developing concrete solutions to the problems looming over the academic...School. In the lab, he uses synthetic biology to create tools to better understand human disease.  Outside...

...particularly given that this has precipitated increases to the NIH recommended pay scale – used by many...while this is a move in the right direction, the increase notably still does not meet the suggested postdoc...this area, keeping the pressure on for further increases will be challenging, but will be aided by the ... new strategies designed to cope with current increases. These two rulings along with progress over the...School. In the lab, he uses synthetic biology to create tools to better understand human disease.  Outside...

...grandsons that decreased their likelihood of death from cardiovascular disease but increased their risk of... DNA methylation patterns, either large increases or decreases. Hypermethylation of CpG islands in the... units of the FRET pair together and causes an increase in the FRET signal. Subsequent demethylation separates...

... aaRSs in these processes. To create the antibody toolbox, they screened antibody candidates using a synthetic...fourteen aminoacyl tRNA synthetases (aaRS) have been created, characterized, and validated by the Gräslund lab...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...pathways By Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting proteins...

...detectors for all fluorophores. This allows for the creation of much larger panels, as fluorophores that cannot...they don’t “wash out” the rest of the panel and create spreading error. Conversely, markers with low expression...should not be paired with these dyes, as this would create difficulty when trying to resolve those populations...signature upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical...spectral flow cytometry has the ability to generate incredible amounts of data from a single experiment. Although...

...transfer plasmid at 30 °C instead of 37 °C and then screen for ITR recombination with a SmaI or XmaI restriction...duplicated regions like the ITRs. Restriction digest screening for ITR deletion is still necessary. To save time...mL of my AAV transfer plasmid cultures and then screen with SmaI digests. I freeze the bacterial pellet...how iodixanol purifications works, shows how to create the iodixanol gradient layers, and has some great...master iodixanol AAV purification. If you’re new to creating gradients, practice making the iodixanol layers...

...suppression of background by antibiotic selection. Screening of transformants by colony PCR confirmed the insertion...obtained if any single component was omitted. Screening for successful Golden Gate Assembly All Golden..., Table 1). These improvements (5- to 12-fold increases in the number of transformants while maintaining...to faster assembly workflows that require less screening to confirm the accuracy of assembly. The fidelity...test the limits of DNA assembly approaches with increasingly complex experimental conditions. The ability...

...needs to be compensated out of APC-Cy7. After increasing the compensation values for APC against APC-Cy7...to be shifted towards the x-axis, so you would increase the compensation value for APC. If the population... to be shifted away from the x-axis, you would decrease the compensation value.   Figure 1: Compensation...population needs to be shifted towards an axis, increase the compensation value, whereas if it the population...population needs to be shifted away from an axis, decrease the compensation value. If neither of these helps...