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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 889 results for: ANT;

Showing: 601 - 620 of 889 results

  1. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...continuation of that work, they have found at least two variants that display increased neuronal expression in ...expression in the liver after systemic delivery.  Importantly, this is true in both mice and nonhuman primates...side effects, by allowing the user to select for variants that infect the CNS while selecting against those... knock-in long sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...e.g. first at 2 months, then at 4 months? Or do antibodies become a prohibitive problem? A: Re-administration...for AAV due to the development of neutralizing antibodies in animal models. All of our engineered AAV are...Xinhong Chen, and Miggy Chuapoco, and to all our participants for their time and excellent questions.    ...

  3. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...you're trapped in a pattern of small errors that significantly impacts your experiments. What do you do when... a failed experiment.  For example, I used to constantly underestimate time needed to prep for an experiment...During really stressful days, I would even take advantage of incubation periods to have another short "quiet...maybe especially) suggestions you are initially resistant to. Just like any other experimental approach,...

  4. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...including hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...in peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek ...learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic studies ...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...and indicate why other neuroscience newbies might want to get into the field. Find Optogenetics Plasmids...There were, of course, many companies selling antibodies, microscopes, and dissection tools, but there...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...neuroscientist looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene...

  6. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...enlarge) As we look ahead to the things to come, we wanted to take a look back at some of the contributions...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting... from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope expressing...Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...students and teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you tell...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...preparing local (Massachusetts), economically disadvantaged students for paid summer internships. A summer...Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused on education and education...

  8. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...have that you really want, please let us know! And if you study chromatin and want to start sharing your...daily basis, the lists below provide a snapshot of relevant resources and a good jumping off point. The following...that histone genes frequently have many different variant names, not all of which can be listed here. If ...

  9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...commonly a plasmid and can infer genetic advantages such as antibiotic resistance. Bacterial conjugation...primary mechanism by which bacteria have gained antibiotic resistance and virulence. Scientists have studied...integration site. The integrated phage remains dormant until it is triggered to enter the lytic cycle....

  10. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...into my role, I realized that actually, I didn’t want to go to grad school. But, I didn’t know what else...great fit for my skillset and interests. As such, I want to be able to share more about this nontraditional...order to keep everything running smoothly, you’ll want to be really organized. Often, product managers ...behind a common vision. Use your network to your advantage  As the profession gains more traction in the...

  11. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways... and how these pathways work, as this could be relevant when planning your experiment. Here, we’ll discuss...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

  12. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...these people you meet will review your papers or grants in the future, and don’t be surprised to know that...personally Scientists in your field will know about grants and projects that might help your work Tenure can...they have to give you theirs! Think about how you want to introduce yourself and what you do. You can probably...night poker games at Gordon Conferences). You don’t want to be so exhausted that you can’t get a lot from...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...depending if you want to use wildtype cas9 or double nickase). For example, if you want to target two genomic...et al. (2013) Science paper. For example, if you want to use double nickase, you could express two spacers...the two plasmids together into your cells. If you want to use double nickase, you need to have four spacers...refer to Cong et al. (2013) for more details. One important note is that this ‘split RNA’ design is not as...

  14. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...doing interesting and relevant work in your field! However, there’s no guarantee you’ll get a response...to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...always faster to order from us!) Ask the PI If you want a plasmid for free, you can always reach out to ...

  15. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...in my more recent positions outside of the lab. Granted, I currently work on the Scientific Support Team...thesis as it is for helping your team reach an important milestone at work.   While I was in graduate school...work through it. Ask yourself if you missed any important details. Or challenge yourself to think of more...that this skillset is a part of your toolkit is important. Paired with your other skills, both technical...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...newest CRISPR plasmids available at Addgene. If you want to read more content related to CRISPR technology...making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be effective... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...improve gene editing performance at therapeutically relevant gene targets and expand the application to the...

  17. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...biomedical workforce. Quotes taken directly from participants (summarized in 2) touched upon many problems...better solution would be to reduce the number of entrants into PhD programs to more closely reflect the ...Labor Market. On this topic it is clear that many important questions, areas for informed discussion, debate...Among other involvements, he helped to establish grants through the Sloan foundation for postdoc-related...

  18. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...subsequent conclusions. Being vigilant about these errors is an important part of maintaining the quality...elements, and nucleotide indels or mismatches in significant plasmid elements. Which differences are considered...This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley...

  19. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...undergraduate students (1, 2), but educators face significant challenges in designing suitable projects. In...because the S. cerevisiae deletion strains are ∆ura3 mutants and cannot grow without uracil supplementation ... Students who participate in the course show significant gains in both content knowledge and research ...PMCID: PMC4012490. 4. Hoffman, C.S., Wood, V., & Fantes, P.A. An ancient yeast for young geneticists: A...

  20. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...decision easier. 1. Müller, Sara M., et al. "Quantification of Förster resonance energy transfer by monitoring...monitoring sensitized emission in living plant cells." (2013). PubMed PMID: 24194740. PubMed Central PMCID...) or would perturb the endogenous state that you want to measure (overexpressing a gene or protein after...detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring”) (Meng...
Showing: 601 - 620 of 889 results