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We narrowed to 898 results for: CAN

Showing: 601 - 620 of 898 results

  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...space per user, but, if you need more space, you can sign up for a plan. Best of all, by not using paper...also a very helpful aspect to this app; the user can search by sequence, category of enzyme, or name to..., and structures into one convenient resource. I can safely say that I still have the single and triple...codon list for each amino acid, but, overall AMINO can be beneficial for new researchers or students beginning...your notebook. The files uploaded are saved and cannot be deleted, so there is no worry about misplacing...find an MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet ...quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS book, this is a great resource...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...foods, and fibers. And because working with plants can be challenging, there are a lot of unexplored areas...not food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful...body of knowledge. Organizations like Open Plant can then build on this research and effectively carry... pursuing scientific or business enterprises, he can be found climbing mountains or playing with his two...plant biology is a significant technical obstacle to plant synthetic biology. We cannot build what we do...now synonymous with GM to the point that people cannot imagine any use for GM technology other than to...sales. Flowers that change color throughout the day cannot be developed through conventional breeding; synthetic...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...manuscript needs to be revised before it can be published, this can delay publication and manuscripts may...that the manuscript requires revisions, the authors can publish a new version of the article, while the first...effort) and the scientific community, as the reports can be very informative as to how a paper is received...journals and their scientific communities aim to significantly improve the reproducibility and reliability ...

  4. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...suggestions. Can’t find a plasmid you’re looking for in the repository? Let us know! You can notify us to...plasmid and a publication where we can find the plasmid. While we cannot guarantee that everyone we reach...

  5. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...applications, and the quality of a standard curve can make or break an experiment. Here, we will provide... similar buffers if not, since buffer components can affect the final readout. When preparing a standard...user, equipment, assay incubation time, etc., which can vary even when the same protocol is being followed...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample...lowest and highest concentrations that the curve can accurately measure.  Dynamic range depends on a number...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...town, and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations associated with SpCas9...activation of the fluorophore. The fluorescent signal can be used as an indicator to determine whether the ...its structure and function? For starters, Cas13a can be used to bind and cleave target RNAs, although ...the exciting discovery that some Cas13a orthologs can function in mammalian and plant cells (Abudayyeh ...the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides...From this analysis, they identified 53 potential candidate genes that fell into 3 categories based on the...question: C2c1, C2c2 and C2c3 (short for Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...purified virus. This process can be completed in one long day, or the virus can be stored at 4 °C and buffer...separate into two phases. If this happens, the solution can be cooled and the two phases mixed together. Lastly...This approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months...SYBR green technology or a TaqMan primer/probe set can be used and samples are quantified by comparing to...and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production and...

  8. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...reusable software components that can be incorporated into software. You can think of these libraries as ingredients...educational, and professional backgrounds. Sometimes this can lead to wildly different ideas of how to approach... our software. What this means for us is that we can focus on the meat of the software and not waste time... places, we might be calling it an aliquot. This can lead to confusion as to what the code is supposed...development happening at Addgene under the hood. A significant portion of our development may not be visible...average of 40 minutes to 10 minutes. While users can’t tangibly see these improvements, you are benefited... been updated in several years) or they make significant changes that make it incompatible with our software...

  9. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...channels. Since a Nextflow process can utilize any scripting language that can be executed on Linux, we created...computer, so it safely operates in isolation, and can be easily shared with others without any complicated...process defines which command or script will run and can be thought of as a particular step in the overall...step in our pipeline together.  Since each process can use a unique container, we customized each container...

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... 2021) can help restrict expression to your cell type of interest, while miRNA target sites can be used...retrograde transport in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene... cell type specificity. An on/off genetic switch can be used for expression control. (c) Viral delivery...which is one of a handful of natural serotypes that can cross the BBB. However, these natural serotypes (...Systemic capsids packaging Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse...spatiotemporal control of transgene expression or activity can be achieved using switches, including riboswitches...use two or more AAVs to transduce the same cell, can accommodate larger, modular genetic payloads. Since...

  11. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...fluorescent proteins, careers, and more. These resources can now be found in one place: the Educational Resources...Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific graphics. PS: they’re free... transcriptase and a prime editing guide RNA, it can introduce insertions, deletions, and all possible...

  12. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...of the virus. While this can be useful, if too many neurons are labeled, it can be difficult to accurately...deficient (i.e. they cannot easily spread between cells like natural RABV or HSV can). AAV does not have...inserted electrodes) may be applied, but these methods can have a negative impact on cellular health. Another...Virus (HSV) also migrates through neurons where it can replicate and by doing so spread across several synaptic...immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters for high and neuron-specific...fluoresce in different colors. An individual neuron can then be traced throughout the brain based on its ...Oh et al., 2014). Images yielded from this study can be found on the Allen Mouse Brain Connectivity Atlas...

  13. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... acids, HA is nine), multiple repeats of the tag can recruit multiple frankenbodies to amplify the signal...two guides require two arrays for Cas9, two guides can be streamlined into a single array for Cas12a. Image...histidine tags, are optimized for SDS-PAGE gels and can be detected on Western blots via engineered immunoglobulin...editor allows for base editing, while gene editing can be done via a nuclease-active CasMINI. This small...is highly specific in mammalian cells without significant off-target effects, yet small enough to be packaged...

  14. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...developed split cytosine and adenine base editors that can be reconstituted in vivo to form a protein identical.... Broadening the spectral diversity of GEVIs can enable resolving single action potentials and further...allows for greater reproducibility as scientists can assemble parts from different labs and experimental...proteins controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine...

  15. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors... proteins determine which types of cells a virus can infect. The VSV-G envelope protein enables this virus...BbsI restriction site, a pair of annealed oligos can be cloned to create a chimeric guide RNA. Find pX330...producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as pCMV-dR8.2...

  16. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...mentoring program at your organization, contact me! I can provide a step-by-step, how-to guide to get one started...same time Broadens the network of the participants Can be a lot of fun! Form your own peer mentoring group...interested and willing to commit to the group. The group can be one gender and career stage (e.g. all women, all... mixed for one or both of these criteria. If you can, include people from different labs, departments ... from peer groups meeting at both the Fox Chase Cancer Center and Brandeis University. 5 easy steps to...meet regularly no matter what, even if one person can’t make it. One of the biggest group mentoring predictors...

  17. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...interested in engaging with us on social media, you can find us on Bluesky: @addgene.bsky.social; or @addgene...associated with an official Addgene account. You can reach out to us directly via email at [email protected]... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

  18. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... with the other 11 presents!)  Can't get enough of CRISPR? Neither can we! Subscribe below to get updates...CRISPR in 2021 and useful resources, fear not! You can find all of them here.  On the first day of CRISPR...

  19. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...) are flanked by unique restriction sites so you can swap sequences effortlessly.  We built these plasmids...plasmids for flexibility and speed, so you can design, test, and innovate faster than ever. Plus, we've...fused to a shortened R2Tocc transposon protein, and can "retarget" the transposon to new loci in the genome...recruitment and nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits — single-base... proteins with biotin. The biotinylated proteins can then be isolated and identified with tools like mass...gene expression to specific cell types in the brain can be a challenging — but critical — factor for successful...the IPI Glypican Collection here! Figure 3: General glypican domain structure. Glypicans are proteoglycans...

  20. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...published concurrently in Science have shown CRISPR can treat a genetic disease in a postnatal mouse model...another approach. Since shorter forms of dystrophin can still be functional, exon skipping is a good option...culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing in ...strategies for Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple exons that...’d like to treat with CRISPR. As seen above, DMD can be treated with “one-size-fits-most” NHEJ-mediated...DMD. If the safety and efficacy of this approach can be optimized, DMD could become one of the first diseases...science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton...
Showing: 601 - 620 of 898 results